C-1

Name: C-1
Brand: tocris
SKU: 0543

Catalog # 0543

Datasheet / COA / SDS

Order Information

Bio-Techne Corporation

tel: (612) 379-2956

tel: (800) 343-7475 (free phone)

customercare.na@bio-techne.com

Shipping Information

$35 to United States

Products in stock will be shipped in 1-3 business days unless specified.

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Key Product Details

Description:	Inhibits PKG, PKA and PKC
Alternative Names:	HA 100
Chemical Name:	1-(5-Isoquinolinesulfonyl)piperazine
Purity:	≥99% (HPLC)

View Full Technical Details for C-1

Biological Activity

Protein kinase inhibitor. Inhibits PKG, PKA and PKC (IC₅₀ values are 4, 8 and 12 μM, respectively). Widely used as a ROCK inhibitor. Increases efficiency of human fibroblast reprogramming with PD 0325901 (Cat.No 4192), CHIR 99021 (Cat.No 4432), A 83-01 (Cat.No 2939) and hLIF. Improves survival of human pluripotent stem cells in culture.

Technical Data

M. Wt	277.34
Formula	C₁₃H₁₅N₃O₂S
Storage	Store at RT
Purity	≥99% (HPLC)
CAS Number	84468-24-6
PubChem ID	3545
InChi Key	UPTYCYWTFGTCCG-UHFFFAOYSA-N
Smiles	O=S(=O)(N1CCNCC1)C1=C2C=CN=CC2=CC=C1

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility

	Solvent	Max Conc. mg/mL	Max Conc. mM
Solubility
Solubility	DMSO	27.73	100

Preparing Stock Solutions

The following data is based on the product molecular weight 277.34.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
1 mM	3.61 mL	18.03 mL	36.06 mL
5 mM	0.72 mL	3.61 mL	7.21 mL
10 mM	0.36 mL	1.80 mL	3.61 mL
50 mM	0.07 mL	0.36 mL	0.72 mL

Molarity Calculator

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Reconstitution Calculator

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References

References are publications that support the biological activity of the product.

Gerard Role of protein kinases in stimulation of human polymorphonuclear leukocyte oxidation metabolism by various agonists. J.Clin.Invest. 1986 PMID: 3003155
Yu Efficient feeder-free episomal reprogramming with small molecules. PLoS.One. 2011 PMID: 21390254
Chen Chemically defined conditions for human iPSC derivation and culture. Nat.Methods 2011 PMID: 21478862

C-1