C-1

Name: C-1
Brand: Tocris Bioscience
SKU: 0543

Tocris Bioscience a Bio-Techne Brand

Catalog # 0543

COA / SDS

Discontinued Product

0543 has been discontinued. View all Protein Kinase C Inhibitors products.

Key Product Details

Description:	Inhibits PKG, PKA and PKC
Alternative Names:	HA 100
Chemical Name:	1-(5-Isoquinolinesulfonyl)piperazine
Purity:	≥99% (HPLC)
Molecular Weight:	277.34

View Full Technical Details for C-1

Biological Activity for C-1

C-1 is a protein kinase inhibitor. Inhibits PKG, PKA and PKC (IC₅₀ values are 4, 8 and 12 μM, respectively). Widely used as a ROCK inhibitor. Increases efficiency of human fibroblast reprogramming with PD 0325901 (Cat.No 4192), CHIR 99021 (Cat.No 4432), A 83-01 (Cat.No 2939) and hLIF. Improves survival of human pluripotent stem cells in culture.

Technical Data for C-1

M. Wt	277.34
Formula	C₁₃H₁₅N₃O₂S
Storage	Store at RT
Purity	≥99% (HPLC)
CAS Number	84468-24-6
PubChem ID	3545
InChi Key	UPTYCYWTFGTCCG-UHFFFAOYSA-N
Smiles	O=S(=O)(N1CCNCC1)C1=C2C=CN=CC2=CC=C1

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility for C-1

	Solvent	Max Conc. mg/mL	Max Conc. mM
Solubility
Solubility	DMSO	27.73	100

Preparing Stock Solutions for C-1

The following data is based on the product molecular weight 277.34.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
1 mM	3.61 mL	18.03 mL	36.06 mL
5 mM	0.72 mL	3.61 mL	7.21 mL
10 mM	0.36 mL	1.80 mL	3.61 mL
50 mM	0.07 mL	0.36 mL	0.72 mL

Molarity Calculator

Calculate the mass, volume, or concentration required for a solution.

Mass

Concentration

Volume

M. Wt*

g/mol

*When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and CoA (available online).

Dilution Calculator

Calculate the dilution required to prepare a stock solution.

Concentration 1 (C1)

Volume 1 (V1)

Concentration 2 (C2)

Volume 2 (V2)

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

Volume (to add to vial)

Mass (in vial)

Desired Reconstitution Concentration

References for C-1

References are publications that support the biological activity of the product.

Yu Efficient feeder-free episomal reprogramming with small molecules. PLoS.One. 2011 PMID: 21390254
Chen Chemically defined conditions for human iPSC derivation and culture. Nat.Methods 2011 PMID: 21478862
Gerard Role of protein kinases in stimulation of human polymorphonuclear leukocyte oxidation metabolism by various agonists. J.Clin.Invest. 1986 PMID: 3003155

Citations for C-1

Please visit our Instrument Citation Database to search our collection of scientific articles that feature our instruments.

C-1