BU 224 hydrochloride is a high affinity ligand for the imidazoline I₂ binding site (K_i = 2.1 nM). Putative I₂ antagonist; antagonizes the effects of imidazoline ligands on morphine antinociception. Produces ipsiversive rotational behavior in rats with a full 6-OHDA lesion of the nigrostriatal tract.

Technical Data

M. Wt	233.7
Formula	C₁₂H₁₁N₃.HCl
Storage	Desiccate at RT
CAS Number	205437-64-5
PubChem ID	11957470
InChi Key	DDFHQXAQWZWRSQ-UHFFFAOYSA-N
Smiles	Cl.C1CN=C(N1)C1=NC2=CC=CC=C2C=C1

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility

	Solvent	Max Conc. mg/mL	Max Conc. mM
Solubility
Solubility	DMSO	2.34	10

Preparing Stock Solutions

The following data is based on the product molecular weight 233.7.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
0.1 mM	42.79 mL	213.95 mL	427.90 mL
0.5 mM	8.56 mL	42.79 mL	85.58 mL
1 mM	4.28 mL	21.39 mL	42.79 mL
5 mM	0.86 mL	4.28 mL	8.56 mL

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References

References are publications that support the biological activity of the product.

Hudson Novel selective compounds for the investigation of imidazoline receptors. Ann.N.Y.Acad.Sci. 1999 PMID: 10415900
MacInnes and Dut Locomotor effects of imidazoline I₂-site-specific ligands and monoamine oxidase inhibitors in rats with a unilateral 6-hydroxyDA lesion of the nigrostriatal pathway. Br.J.Pharmacol. 2004 PMID: 15545290
Sanchez-Blasquez Activation of I₂-imidazoline receptors enhances supraspinal mor. analgesia in mice: a model to detect agonist and antagonist activities at these receptors. Br.J.Pharmacol. 2000 PMID: 10781010
Hudson Affinity and selectivity of BU224 and BU239 for rabbit brain non-adrenoceptor idazoxan binding sites (I₂) sites). Br.J.Pharmacol.

Citations (2)

Showing 1 - 2 of 2 citations Showing All

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Citations are publications that use Bio-Techne products. Selected citations for BU 224 hydrochloride include:

Gründemann et al. (2002) Activation of the extraneuronal monoamine transporter (EMT) from rat expressed in 293 cells. Br J Pharmacol. 137:910
PMID: 12411423
Sánchez-Blázquez et al. (2000) Activation of I(2)-imidazoline receptors enhances supraspinal mor. analgesia in mice: a model to detect agonist and antagonist activities at these receptors. Br J Pharmacol. 130:146
PMID: 10781010

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Product Datasheets

COA

Certificate of Analysis

To download a Certificate of Analysis, please select a batch below.

BU 224 hydrochloride

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Key Product Details

Biological Activity