API-1

Tocris Bioscience a Bio-Techne Brand

Catalog # 3897

Citations (4)

(1)

COA / SDS

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Key Product Details

Description:	Selective Akt/PKB inhibitor. Antitumor
Chemical Name:	4-Amino-5,8-dihydro-5-oxo-8-β-D-ribofuranosyl-pyrido[2,3-d]pyrimidine-6-carboxamide
Purity:	≥96% (HPLC)
Molecular Weight:	337.29

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Biological Activity for API-1

API-1 is an Akt/protein kinase B (PKB) inhibitor. Binds the pleckstrin homology domain of Akt and blocks Akt membrane translocation. Inhibits EGF-induced kinase activity of Akt1, Akt2 and Akt3. Induces cell growth arrest and apoptosis in human cancer cells expressing constitutively active Akt. Displays antitumor activity in vitro and in vivo.

Technical Data for API-1

M. Wt	337.29
Formula	C₁₃H₁₅N₅O₆
Storage	Store at RT
Purity	≥96% (HPLC)
CAS Number	36707-00-3
PubChem ID	24773090
InChi Key	SPBWHPXCWJLQRU-FITJORAGSA-N
Smiles	O[C@@H]1[C@@H](CO)O[C@@H](N2C(N=CN=C3N)=C3C(C(C(N)=O)=C2)=O)[C@@H]1O

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility for API-1

	Solvent	Max Conc. mg/mL	Max Conc. mM
Solubility
Solubility	DMSO	33.73	100

Preparing Stock Solutions for API-1

The following data is based on the product molecular weight 337.29.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
1 mM	2.96 mL	14.82 mL	29.65 mL
5 mM	0.59 mL	2.96 mL	5.93 mL
10 mM	0.30 mL	1.48 mL	2.96 mL
50 mM	0.06 mL	0.30 mL	0.59 mL

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References for API-1

References are publications that support the biological activity of the product.

Kim A small molecule inhibits Akt through direct binding to Akt and preventing Akt membrane translocation. J.Biol.Chem. 2010 PMID: 20068047

Citations for API-1 (5)

Showing 1 - 4 of 5 citations Showing All

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Citations are publications that use Bio-Techne products. Selected citations for API-1 include:

Jezierska-Drutel et al. (2019) The peroxidase PRDX1 inhibits the activated phenotype in mammary fibroblasts through regulating c-Jun N-terminal kinases. BMC Cancer. 19:812
PMID: 31419957
Arslan and Yerer (2018) α-Chaconine and α-Solanine Inhibit RL95-2 Endometrium Cancer Cell Proliferation by Reducing Expression of Akt (Ser473) and ERα (Ser167). Nutrients. 10
PMID: 29799481
Shamblott et al. (2016) Neurogenin 3 is regulated by neurotrophic tyrosine kinase receptor type 2 (TRKB) signaling in the adult human exocrine pancreas Cell Communication and Signaling. 14:23
PMID: 27659207
Shamblott et al. (2016) Neurogenin 3 is regulated by neurotrophic tyrosine kinase receptor type 2(TRKB) signaling in the adult human exocrine pancreas. Cell Communication and Signaling. 14:23
PMID: 27659207
Zhu et al. (2012) BlyS is up-regulated by hypoxia and promotes migration of human breast cancer cells. EMBO J. 31:31
PMID: 22463935

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Study of API-1 induction c-FLIP degradation and synergization with TRAIL to augment apoptosis

Name: Anonymous

Species: Human

Study of API-1 induction c-FLIP degradation and synergization with TRAIL to augment apoptosis.API-1 effectively induced apoptosis in tested cancer cell lines including activation of caspase-8 and caspase-9.

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Product Documents for API-1

COA

Certificate of Analysis

To download a Certificate of Analysis, please select a batch below.