AG 555

Catalog # 0618

Datasheet / COA / SDS

Discontinued Product

0618 has been discontinued. View all EGF Receptor Inhibitors products.

Order Information

Bio-Techne Corporation

tel: (800) 343-7475 (free phone)

tel: (612) 379-2956

customercare.na@bio-techne.com

Shipping Information

$35 to United States

Products in stock will be shipped in 1-3 business days unless specified.

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Key Product Details

Description:	Potent EGFR-kinase inhibitor
Alternative Names:	Tyrphostin AG 555
Chemical Name:	(E)-2-Cyano-3-(3,4-dihydroxyphenyl)-N-(3-phenylpropyl)-2-propenamide
Purity:	≥99% (HPLC)
Molecular Weight:	322.36

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Biological Activity

AG 555 is a potent epidermal growth factor receptor (EGFR) kinase inhibitor (IC₅₀ = 0.7 μM) that displays 50-fold and >140-fold selectivity over ErbB2 and insulin receptor kinase respectively. Induce G₁ growth arrest selectively in transformed cells (IC₅₀ values are 6.4 and 9.4 μM in HPV16-immortalized and normal keratinocytes respectively).

Technical Data

M. Wt	322.36
Formula	C₁₉H₁₈N₂O₃
Storage	Desiccate at +4°C
Purity	≥99% (HPLC)
CAS Number	133550-34-2
PubChem ID	5328770
InChi Key	GSQOBTOAOGXIFL-LFIBNONCSA-N
Smiles	OC1=CC=C(\C=C(/C#N)C(=O)NCCCC2=CC=CC=C2)C=C1O

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility

	Solvent	Max Conc. mg/mL	Max Conc. mM
Solubility
Solubility	DMSO	9.67	30

Preparing Stock Solutions

The following data is based on the product molecular weight 322.36.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
0.3 mM	10.34 mL	51.70 mL	103.40 mL
1.5 mM	2.07 mL	10.34 mL	20.68 mL
3 mM	1.03 mL	5.17 mL	10.34 mL
15 mM	0.21 mL	1.03 mL	2.07 mL

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References

References are publications that support the biological activity of the product.

Ben-Bassat Tyrphostins that suppress the growth of human papilloma virus 16-immortalized human keratinocytes. J.Pharmacol.Exp.Ther. 1999 PMID: 10454524
Gazit Tyrphostins. 2. Heterocyclic and α-substituted benzylidenemalononitrile tyrophostins as potent inhibitors of EGF receptor and ErbB2/neu tyrosine kinases. J.Med.Chem. 1991 PMID: 1676428
Levitzki and Gazit Tyrosine kinase inhibition: an approach to drug development. Science 1995 PMID: 7892601