AG 494

Tocris Bioscience a Bio-Techne Brand

Catalog # 0619

COA / SDS

Discontinued Product

0619 has been discontinued. View all EGF Receptor Inhibitors products.

Key Product Details

Description:	Potent EGFR-kinase inhibitor
Chemical Name:	(E)-2-Cyano-3-(3,4-dihydroxyphenyl)-N-phenyl-2-propenamide
Molecular Weight:	280.28

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Biological Activity for AG 494

AG 494 is a potent inhibitor of epidermal growth factor receptor (EGFR) kinase (IC₅₀ = 0.7 μM). Selective over ErbB2, PDGFR and insulin receptor kinase (IC₅₀ values are 42, 6 and > 100 μM respectively).

Technical Data for AG 494

M. Wt	280.28
Formula	C₁₆H₁₂N₂O₃
Storage	Desiccate at +4°C
CAS Number	133550-35-3
PubChem ID	5328771
InChi Key	HKHOVJYOELRGMV-XYOKQWHBSA-N
Smiles	OC1=C(O)C=C(\C=C(/C#N)C(=O)NC2=CC=CC=C2)C=C1

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility for AG 494

	Solvent	Max Conc. mg/mL	Max Conc. mM
Solubility
	ethanol	1.4	5
	DMSO	14.01	50

Preparing Stock Solutions for AG 494

The following data is based on the product molecular weight 280.28.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
0.5 mM	7.14 mL	35.68 mL	71.36 mL
2.5 mM	1.43 mL	7.14 mL	14.27 mL
5 mM	0.71 mL	3.57 mL	7.14 mL
25 mM	0.14 mL	0.71 mL	1.43 mL

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Reconstitution Calculator

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References for AG 494

References are publications that support the biological activity of the product.

Tomlins Cross-talk between the calcium-sensing receptor and the epidermal growth factor receptor in Rat-1 fibroblasts. Exp.Cell Res. 2005 PMID: 15950968
Ben-Bassat Tyrphostins that suppress the growth of human papilloma virus 16-immortalized human keratinocytes. J.Pharmacol.Exp.Ther. 1999 PMID: 10454524
Gazit Tyrphostins. 2. Heterocyclic and α-substituted benzylidenemalononitrile tyrophostins as potent inhibitors of EGF receptor and ErbB2/neu tyrosine kinases. J.Med.Chem. 1991 PMID: 1676428

Citations for AG 494

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