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Key Product Details

Species Reactivity

Sheep

Applications

Flow Cytometry, Immunocytochemistry, Immunohistochemistry

Label

NL493 (Excitation = 493 nm, Emission = 514 nm)

Antibody Source

Polyclonal Donkey IgG

Product Summary for Sheep IgG NorthernLights™ NL493-conjugated Antibody

Immunogen

Sheep IgG

Specificity

Sheep IgG (H+L)

Clonality

Polyclonal

Host

Donkey

Isotype

IgG

Scientific Data Images for Sheep IgG NorthernLights™ NL493-conjugated Antibody

Detection of Sheep IgG Primary Antibody by Immunocytochemistry.

Detection of human AIF by incubating cells with 5 μg/mL of Sheep anti-Human AIF polyclonal antibody (AF5824) at room temperature for 1 hour followed by incubation with 1:200 dilution of Donkey Anti-Sheep IgG NorthernLights™ NL493-conjugated secondary antibody for 1 hour at room temperature. Note strong positive staining in KG-1 cells (left panel, positive control) and a lack of non-specific background staining in the absence of primary antibodies (right panel, negative control).

Applications for Sheep IgG NorthernLights™ NL493-conjugated Antibody

Application
Recommended Usage

Flow Cytometry

Use at a 1:200 dilution

Immunocytochemistry

Use at a 1:200 dilution

Immunohistochemistry

Use at a 1:200 dilution
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 1 review rated 3 using NL012 in the following applications:

Published Applications

Read 6 publications using NL012 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.
*Contains <0.1% Sodium Azide, which is not hazardous at this concentration according to GHS classifications. Refer to the Safety Data Sheet (SDS) for additional information and handling instructions.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8° C. Do not use past expiration date. Protect from light.

Background: IgG

R&D Systems offers a range of secondary antibodies and controls for flow cytometry, immunohistochemistry, and Western blotting. We provide species-specific secondary antibodies that are available with a variety of conjugated labels.Our NorthernLights fluorescent secondary antibodies are bright and resistant to photobleaching. We are currently offering secondary antibodies recognizing mouse, rat, goat, sheep, and rabbit IgG as well as chicken IgY. These reagents are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy.

Long Name

Immunoglobulin G

Alternate Names

Immunoglobulin G, ImmunoglobulinG

Product Documents for Sheep IgG NorthernLights™ NL493-conjugated Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Sheep IgG NorthernLights™ NL493-conjugated Antibody

For research use only

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FAQs for Sheep IgG NorthernLights™ NL493-conjugated Antibody

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  • Q: Are products derived from donkey serum listed on the Convention on International Trade in Endangered Species of Wild Fauna and Flora (CITES)?

    A: All products derived from donkeys were produced from the sera of domestic donkeys of a genus and species not protected by CITES. No CITES permit is required for the transportation of these materials.

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