Recombinant Mouse TGF-beta RI/ALK-5 Fc Chimera Protein, CF

Name: Recombinant Mouse TGF-beta RI/ALK-5 Fc Chimera Protein, CF
Brand: R&D Systems
SKU: 587-RI

R&D Systems, Inc. a Bio-Techne Brand

Catalog # 587-RI

Citations (2)

Product Datasheet / COA / SDS

This item is in process of being discontinued.

Catalog #

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Key Product Details

Source:	Sf 21 (baculovirus)
Accession #:	BAA05023
Structure / Form:	Disulfide-linked homodimer
Applications:	Bioactivity

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Product Specifications for Recombinant Mouse TGF-beta RI/ALK-5 Fc Chimera Protein, CF

Source

Spodoptera frugiperda, Sf 21 (baculovirus)-derived mouse TGF-beta RI/ALK-5 protein

Mouse TGF-beta RI (Ala21-Glu121) & (Thr22-Glu121) Accession # BAA05023	IEGRMD	Human IgG₁ (Pro100-Lys330)
N-terminus		C-terminus

Purity

>90%, by SDS-PAGE under reducing conditions and visualized by silver stain

Endotoxin level

<0.1 EU per 1 μg of the protein by the LAL method.

N-terminal sequence Analysis

Ala21 & Thr22

Predicted Molecular Mass

37.5 kDa (monomer)

SDS-PAGE

40-50 kDa, reducing conditions

Activity

Measured by its binding ability in a functional ELISA. When Recombinant Rat Endoglin/CD105 Fc Chimera (Catalog # 6440-EN) or Recombinant Mouse Endoglin/CD105 Fc Chimera (Catalog # 1320-EN) is immobilized at 2 μg/mL (100 μL/well), the concentration of Recombinant Mouse TGF-beta RI/ALK-5 Fc Chimera that produces 50% of the optimal binding response is found to be approximately 0.25-1.25 μg/mL.

Formulation, Preparation and Storage

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

587-RI

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 500 μg/mL in sterile PBS. Reconstitution Buffer Available: Size / Price Qty Loading... RB01:
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: TGF-beta RI/ALK-5

TGF-beta RI, also called ALK-5, is an approximately 55 kDa type I transmembrane serine/threonine receptor kinase (1, 2). It contains a cysteine-rich extracellular domain (ECD), a transmembrane helix, and a C-terminal cytoplasmic kinase domain (3). Within the cytoplasmic domain there is also a short, conserved regulatory sequence known as the GS region that is N-terminal to the kinase domain (1). Within the ECD, mouse TGF-beta RI shares 98% and 90% amino acid sequence identity with rat and human TGF-beta RI, respectively. In the presence of TGF-beta, TGF-beta RI forms a complex with, and is phosphorylated by, TGF-beta RII (1). Phosphorylated TGF-beta RI can then transiently bind and phosphorylate Smad2 and Smad3 (2, 4-6). These phosphorylated Smads form heteromeric complexes with Smad4, translocate to the nucleus, and regulate target gene transcription (2, 4-6). TGF-beta RI is likely important during development, since mice deficient for TGF-beta RI die at midgestation with severe defects in vascular development of the yolk sac and placenta, and an absence of circulating red blood cells (7). Furthermore, TGF-beta RI appears to be involved in proper lymphatic network development (8). Mutations in TGF-beta RI have been identified in pancreatic, colorectal, ovarian, and head and neck cancers (9).

Item	Value
References	Wrana, J.L. et al. (1994) Nature 370:341. Massagué, J. (2012) Nat. Rev. Mol. Cell Biol. 13:616. Huse, M. et al. (1999) Cell 96:425. Marcías-Silva, M. et al. (1996) Cell 87:1215. Zhang, Y. et al. (1996) Nature 383:168. Huse, M. et al. (2001) Mol. Cell 8:671. Larsson, J. et al. (2001) EMBO J. 20:1663. James, J.M. et al. (2013) Development 140:3903. Sheen, Y.Y. et al. (2013) Biomol. Ther. (Seoul) 21:323.
Long Name	Transforming Growth Factor beta Receptor I
Entrez Gene IDs	7046 (Human); 21812 (Mouse); 29591 (Rat)
Alternate Names	AAT5, ACVRLK4, ALK-5, ALK-5ALK5, Activin receptor-like kinase 5, EC 2.7.11, EC 2.7.11.30, LDS1A, LDS2A, SKR4, Serine/threonine-protein kinase receptor R4, TGF-bRI, TGF-beta RI, TGF-beta receptor type I, TGF-beta receptor type-1, TGF-beta type I receptor, TGFB1R1, TGFBR1, TGFR-1, TGFbetaRI, Transforming growth factor-beta receptor type I, activin A receptor type II-like kinase, 53kD, activin A receptor type II-like kinase, 53kDa, tbetaR-I, transforming growth factor beta receptor I, transforming growth factor, beta receptor 1, transforming growth factor, beta receptor I (activin A receptor type II-likekinase, 53kD)

Citations for Recombinant Mouse TGF-beta RI/ALK-5 Fc Chimera Protein, CF (2)

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Citations are publications that use Bio-Techne products. Selected citations for Recombinant Mouse TGF-beta RI/ALK-5 Fc Chimera Protein, CF include:

Species: Human
Sample Types: Recombinant Protein
Applications: Surface Plasmon Resonance

Castonguay R et al. (2011), Soluble Endoglin Specifically Binds Bone Morphogenetic Proteins 9 and 10 via Its Orphan Domain, Inhibits Blood Vessel Formation, and Suppresses Tumor Growth. J. Biol. Chem., 286(34):30034-46.
PMID: 21737454
Species: N/A
Sample Types: N/A
Applications: ELISA (Standard)

Niessen K et al. (2010), ALK1 signaling regulates early postnatal lymphatic vessel development. Blood, 115(8):1654-61.
PMID: 19903896

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