Recombinant Mouse Semaphorin 3E Fc Chimera Protein, CF

Name: Recombinant Mouse Semaphorin 3E Fc Chimera Protein, CF
Brand: R&D Systems
SKU: 3238-S3

R&D Systems, Inc. a Bio-Techne Brand

Catalog # 3238-S3

Citations (5)

Product Datasheet / COA / SDS

Discontinued Product

3238-S3 has been discontinued. View all Semaphorin 3E products.

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Key Product Details

Source:	Sf 21 (baculovirus)
Accession #:	P70275
Structure / Form:	Disulfide-linked homodimer
Applications:	Bioactivity

View Full Technical Details for Recombinant Mouse Semaphorin 3E Fc Chimera Protein, CF

Product Specifications for Recombinant Mouse Semaphorin 3E Fc Chimera Protein, CF

Source

Spodoptera frugiperda, Sf 21 (baculovirus)-derived mouse Semaphorin 3E protein

Mouse Semaphorin 3E Asn27-Phe766 (Arg557Ala and Arg560Ala) Accession # P70275	IEGRMD	Human IgG₁ (Pro100-Lys330)
N-terminus		C-terminus

Purity

>90%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin level

<1.0 EU per 1 μg of the protein by the LAL method.

N-terminal sequence Analysis

Asn27

Predicted Molecular Mass

112 kDa (monomer)

SDS-PAGE

110-120 kDa, reducing conditions

Activity

Measured by its ability to cause collapse of chick embryonic dorsal root ganglia (DRG) neuron growth cones. 2.5‑5.0 µg/mL of Recombinant Mouse Semaphorin 3E Fc Chimera causes >50% growth cone collapse in the presence of 10 ng/mL of Recombinant Human beta ‑NGF (Catalog # 256-GF).

Formulation, Preparation and Storage

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

3238-S3

Formulation	Lyophilized from a 0.2 μm filtered solution in Tris, NaCl and Tween® 20.
Reconstitution	Reconstitute at 100 μg/mL in sterile PBS. Reconstitution Buffer Available: Size / Price Qty Loading... RB01:
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Semaphorin 3E

Semaphorin 3E (Sema3E), previously known as SemaH, is one of six Class 3 (secreted) semaphorins which function in axon guidance and/or vascular tip cell guidance during development (1). Sema3E contains a seven-blade beta -propeller sema domain, a cysteine-knot PSI domain, an Ig-like domain, and a basic region. Dimerization and cleavage within the basic region are required for the repulsing activity of class 3 semaphorins (2). Sema3E can also be cleaved at a furin consensus sequence C-terminal to the sema domain, resulting in a 61 kDa form that does not dimerize and is highly expressed in tumor cell lines with metastatic potential (3, 4). Mature mouse Sema3E shares 90% and 98% aa sequence identity with human and rat Sema3E, respectively. Sema3E signaling is transduced by Plexin D1 which may also be associated with Neuropilin 1 and/or VEGF R2 (2, 5, 6). Its interaction with Plexin D1 inhibits axon migration in the neocortex and forebrain (6, 7), although it can attract axons that express both Plexin D1 and Neuropilin 1 (6). Sema3E promotes axonal growth (5), the development of glutamatergic synaptic specificity (8, 9), and the development of GnRH producing neurons (10). Genetic disruption of either Sema3E or Plexin D1 in mouse causes excessive and disorganized vascular growth and branching, indicating the importance of this ligand-receptor pair for vascular guidance (11, 12). In addition, Sema3E is up-regulated by inflammatory macrophages and damaged hepatocytes (13-15). It inhibits smooth muscle cell proliferation and migration in the asthmatic airway (16), promotes hepatic stellate cell activation and wound healing (15), and regulates the migration of developing thymocytes (17).

Item	Value
References	Oh, W.J. and C. Gu (2013) Semin. Cell Dev. Biol. 24:156. Adams, R. H. et al. (1997) EMBO J. 16:6077. Christensen, C. et al. (2005) Cancer Res. 65:6167. Casazza, A. et al. (2010) J. Clin. Invest. 120:2684. Bellon, A. et al. (2010) Neuron 66:205. Chauvet, S. et al. (2007) Neuron 56:807. Bribian, A. et al. (2014) Nat. Commun. 5:4265. Ding, J.B. et al. (2011) Nat. Neurosci. 15:215. Pecho-Vrieseling, E. et al. (2009) Nature 459:842. Cariboni, A. et al. (2015) J. Clin. Invest. 125:2413. Gu, C. et al. (2005) Science 307:265. Gitler, A. D. et al. (2004) Developmental Cell 7:107. Wanschel, A. et al. (2013) Arterioscler. Thromb. Vasc. Biol. 33:886. Shimizu, I. et al. (2013) Cell Metab. 18:491. Yagai, T. et al. (2014) Am. J. Pathol. 184:2250. Movassagh, H. et al. (2014) J. Allergy Clin. Immunol. 133:560. Choi, Y.I. et al. (2014) Proc. Natl. Acad. Sci. USA 111:379.
Entrez Gene IDs	9723 (Human); 20349 (Mouse)
Alternate Names	(semaphorin) 3E, KIAA0331M-SEMAH, SEMAH, SEMAHM-SemaK, Sema3E, Semaphorin 3E, coll-5, sema domain, immunoglobulin domain (Ig), short basic domain, secreted, semaphorin-3E

Citations for Recombinant Mouse Semaphorin 3E Fc Chimera Protein, CF (5)

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Citations are publications that use Bio-Techne products. Selected citations for Recombinant Mouse Semaphorin 3E Fc Chimera Protein, CF include:

Species: Bovine
Sample Types: Cells
Applications: Cell Culture

V Mehta et al. (2020), The guidance receptor plexin D1 is a mechanosensor in endothelial cells Nature, 578(7794):290-295.
PMID: 32025034
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

K Burk et al. (2017), Post-endocytic sorting of Plexin-D1 controls signal transduction and development of axonal and vascular circuits Nat Commun, 8(0):14508.
PMID: 28224988
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

Yagai T et al. (2014), Semaphorin 3E secreted by damaged hepatocytes regulates the sinusoidal regeneration and liver fibrosis during liver regeneration. Am J Pathol, 184(8):2250-9.
PMID: 24930441
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

Wanschel et al. (2013), Neuroimmune guidance cue Semaphorin 3E is expressed in atherosclerotic plaques and regulates macrophage retention. Arterioscler Thromb Vasc Biol, 33(5):886-93.
PMID: 23430613
Species: Rat
Sample Types: Whole Cells
Applications: Bioassay

Uesugi K et al. (2009), Different requirement for Rnd GTPases of R-Ras GAP activity of Plexin-C1 and Plexin-D1. J. Biol. Chem., 284(11):6743-51.
PMID: 19136556

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Showing 1 - 5 of 5 citations Showing All

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Recombinant Mouse Semaphorin 3E Fc Chimera Protein, CF

R&D Systems, Inc. a Bio-Techne Brand

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