Recombinant Mouse Noggin Fc Chimera Protein, CF

Name: Recombinant Mouse Noggin Fc Chimera Protein, CF
Brand: R&D Systems
SKU: 719-NG

R&D Systems, Inc. a Bio-Techne Brand

Catalog # 719-NG

Citations (18)

Product Datasheet / COA / SDS

New Version Available - 6997-NG/CF

A New rmNoggin is Now Available! It has ~2 fold better activity, lower endotoxin specifications, and no tag!

Catalog #

Availability

Size / Price

Qty

Setting the standard in quality research reagents

Key Product Details

Source:	NS0
Accession #:	U79163
Structure / Form:	Disulfide-linked homodimer
Applications:	Bioactivity

View Full Technical Details for Recombinant Mouse Noggin Fc Chimera Protein, CF

Complete Your Research

Product Specifications for Recombinant Mouse Noggin Fc Chimera Protein, CF

Source

Mouse myeloma cell line, NS0-derived mouse Noggin protein

Mouse Noggin (Leu20-Cys232) Accession # U79163	IEGRMD	Human IgG₁ (Pro100-Lys330)
N-terminus		C-terminus

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal sequence Analysis

Leu20

Predicted Molecular Mass

50 kDa (monomer)

SDS-PAGE

55-60 kDa, reducing conditions

Activity

Measured by its ability to inhibit BMP-4-induced alkaline phosphatase production by ATDC5 mouse chondrogenic cells. The ED₅₀ for this effect is 0.06-0.3 µg/mL in the presence of 30 ng/mL of Recombinant Human BMP-4 (Catalog # 314-BP).

Scientific Data Examples for Recombinant Mouse Noggin Fc Chimera Protein, CF

	Recombinant Mouse Noggin Fc Chimera Protein Bioactivity Measured by its ability to inhibit BMP-4-induced alkaline phosphatase production by ATDC5 mouse chondrogenic cells. The ED₅₀ for this effect is 0.06-0.3 µg/mL in the presence of 30 ng/mL of Recombinant Human BMP-4 (314-BP).

Formulation, Preparation and Storage

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

719-NG

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS and EDTA.
Reconstitution	Reconstitute at 100 μg/mL in sterile PBS. Reconstitution Buffer Available: Size / Price Qty Loading... RB01:
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

Volume (to add to vial)

Mass (in vial)

Desired Reconstitution Concentration

Background: Noggin

Noggin was originally cloned based on its dorsalizing activity in Xenopus embryos. Mammalian noggins were subsequently identified and cloned from human, mouse and rat cDNA libraries. Mouse noggin cDNA encodes a 232 amino acid (aa) residue precursor protein with 19 aa residue putative signal peptide that is cleaved to generate the 213 aa residue mature protein which is secreted as a homodimeric glycoprotein. Noggin is a highly conserved molecule. Mature mouse noggin shares 99% and 83% aa sequence identity with human and Xenopus noggin, respectively. Noggin has a complex pattern of expression during embryogenesis. In the adult, noggin is expressed in the central nervous system and in several adult peripheral tissues such as lung, skeletal muscle and skin. Noggin has been shown to be a high-affinity BMP (bone morphogenetic protein) binding protein that antagonizes BMP bioactivities.

Item	Value
References	Smith, W.C. and R.M. Harland (1992) Cell 70:829. Valenzuela, D.M. et al. (1995) J. Neurosci. 15:6077. Brunet, L.J. et al. (1998) Science 280:1455.
Entrez Gene IDs	9241 (Human); 18121 (Mouse)
Alternate Names	NOG, Noggin, SYM1, SYNS1, SYNS1A, symphalangism 1 (proximal), synostoses (multiple) syndrome 1

Citations for Recombinant Mouse Noggin Fc Chimera Protein, CF (18)

Showing 1 - 10 of 18 citations Showing All

Filter By:

Search Title:

Citations are publications that use Bio-Techne products. Selected citations for Recombinant Mouse Noggin Fc Chimera Protein, CF include:

Species: Human
Sample Types: Whole Cells
Applications: Bioassay

S Ouahoud et al. (2021), Kinome-wide analysis of the effect of statins in colorectal cancer British Journal of Cancer, 0(0).
PMID: 33742146
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

P Bergström et al. (2021), Herpes Simplex Virus 1 and 2 Infections during Differentiation of Human Cortical Neurons Viruses, 13(10).
PMID: 34696502
Species: Chicken
Sample Types: Whole Cells
Applications: Bioassay

BA Boswell et al. (2017), Dual function of TGF? in lens epithelial cell fate: implications for secondary cataract Mol. Biol. Cell, 0(0).
PMID: 28209733
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

(2016), Amyloid precursor protein expression and processing are differentially regulated during cortical neuron differentiation Sci Rep, 6(0):29200.
PMID: 27383650
Species: Mouse
Sample Types: Whole Tissue
Applications: Bioassay

Sengle G et al. (2015), Abnormal Activation of BMP Signaling Causes Myopathy in Fbn2 Null Mice. PLoS Genet, 11(6):e1005340.
PMID: 26114882
Species: Mouse
Sample Types: In Vivo

Ueki Y et al. (2015), A transient wave of BMP signaling in the retina is necessary for Muller glial differentiation. Development, 142(3):533-43.
PMID: 25605781
Species: Mouse
Sample Types: Embryo
Applications: In vivo

Yamamoto-Shiraishi Y et al. (2013), Wnt and BMP signaling cooperate with Hox in the control of Six2 expression in limb tendon precursor. , 0(0).
PMID: 23499659
Species: Chicken
Sample Types: Whole Cells
Applications: Bioassay

Boswell BA et al. (2008), Essential role of BMPs in FGF-induced secondary lens fiber differentiation. Dev. Biol., 324(2):202-12.
PMID: 18848538
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Shiraki N et al. (2008), Guided differentiation of embryonic stem cells into Pdx1-expressing regional-specific definitive endoderm. Stem Cells, 26(4):874-85.
PMID: 18238854
Species: Chicken
Sample Types: Whole Tissue
Applications: Bioassay

Boswell BA et al. (2008), Cross-talk between fibroblast growth factor and bone morphogenetic proteins regulates gap junction-mediated intercellular communication in lens cells. Mol. Biol. Cell, 19(6):2631-41.
PMID: 18400943
Species: Bovine
Sample Types: Whole Cells
Applications: Bioassay

Yao Y et al. (2007), Activin-like kinase receptor 1 (ALK1) in atherosclerotic lesions and vascular mesenchymal cells. Cardiovasc. Res., 74(2):279-89.
PMID: 17074310
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

Lindsley RC et al. (2006), Canonical Wnt signaling is required for development of embryonic stem cell-derived mesoderm. Development, 133(19):3787-96.
PMID: 16943279
Species: Xenopus
Sample Types: In Vivo
Applications: In Vivo

Delaune E et al. (2005), Neural induction in Xenopus requires early FGF signalling in addition to BMP inhibition. Development, 132(2):299-310.
PMID: 15590738
Species: Xenopus
Sample Types: In Vivo
Applications: In Vivo

Reversade B et al. (2005), Regulation of ADMP and BMP2/4/7 at opposite embryonic poles generates a self-regulating morphogenetic field. Cell, 123(6):1147-60.
PMID: 16360041
Species: Xenopus
Sample Types: In Vivo
Applications: In Vivo

Holzschuh J et al. (2005), Requirements for endoderm and BMP signaling in sensory neurogenesis in zebrafish. Development, 132(16):3731-42.
PMID: 16077092
Species: Chicken
Sample Types: In Vivo
Applications: In Vivo

Chang W et al. (2004), The development of semicircular canals in the inner ear: role of FGFs in sensory cristae. Development, 131(17):4201-11.
PMID: 15280215
Species: Xenopus
Sample Types: In Vivo
Applications: In Vivo

Wacker SA et al. (2004), Timed interactions between the Hox expressing non-organiser mesoderm and the Spemann organiser generate positional information during vertebrate gastrulation. Dev. Biol., 268(1):207-19.
PMID: 15031117
Species: Rat
Sample Types: In Vivo
Applications: In Vivo

Chang CF et al. (2002), Bone morphogenetic proteins are involved in fetal kidney tissue transplantation-induced neuroprotection in stroke rats. Alcohol and HIV-Derived Hepatocyte Apoptotic Bodies Induce Hepatic Stellate Cell Activation, 43(3):418-26.
PMID: 12243771

There are no citations that match your criteria.

Showing 1 - 10 of 18 citations Showing All

Product Documents for Recombinant Mouse Noggin Fc Chimera Protein, CF

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS