Skip to main content

Novus Biologicals products are now on bio-techne.com

Better as one

Recombinant Mouse Granzyme D Protein, CF

Bio-Techne includes R&D Systems | Catalog # 1365-SE

Catalog #
Availability
Size / Price
Qty
Loading...
1365-SE-010

Key Product Details

Source

NS0

Accession #

Conjugate

Unconjugated

Applications

Enzyme Activity

Product Specifications

Source

Mouse myeloma cell line, NS0-derived mouse Granzyme D protein
Ile21-Leu252 with a C-terminal 10-His tag

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<1.0 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Ile21 & His25

Predicted Molecular Mass

27 kDa

SDS-PAGE

42 kDa, reducing conditions

Activity

Measured by its ability to cleave a peptide substrate, Succinyl-Phe-Leu-Phe-ThioBenzyl ester (Suc-FLF-SBzl), in the presence of 5,5’-Dithio-bis (2-nitrobenzoic acid) (DTNB). Edwards, K.M. et al. (1999) J. Biol. Chem. 274:30468.
The specific activity is >50 pmol/min/µg, as measured under the described conditions.

Formulation, Preparation and Storage

1365-SE
Formulation Lyophilized from a 0.2 μm filtered solution in HEPES and NaCl.
Reconstitution Reconstitute at 100 μg/mL in sterile 25 mM HEPES and 200 mM NaCl, pH 7.5.
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -20 to -70 °C as supplied.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Granzyme D

Granzyme D is a member of the granzyme family of the serine proteases found specifically in the cytotoxic granules of cytotoxic T lymphocytes (CTL) and natural killer (NK) cells (1-3). Granzyme D’s functions are largely unknown. However, its selective expression in primary and functional hematopoietic stromal lines correlates with capacity of supporting growth of a pre-T lymphoma clone (4). Together with Granzymes E, F and G, it is regulated through pregnancy and by IL-2 and IL-15 in granulated metrial gland cells (5). The human or rat counterpart of mouse Granzyme D has not been found. Like other granzymes, mouse Granzyme D is not secreted as a zymogen but stored as a fully processed and activated enzyme in the cytoplasmic granules of CTL (1). It is synthesized as a 252 amino acid precursor with an 18 amino acid signal peptide and a 2 amino acid propeptide (1). The mature protein (residues 21-252) is expressed and purified.

References

  1. Jenne, D.E. et al. (1988) Proc. Natl. Acad. Sci. USA 85:4814.
  2. Kam, C.-M. et al. (2000) Biochim. Biophys. Acta 1477:307.
  3. Smyth, M.J. et al. (1996) J. Leukoc. Biol. 60:555.
  4. Yokoi, A. et al. (1999) Biochem. Biophys. Res. Comm. 264:768.
  5. Allen, M.P. and M. Nilsen-Hamilton (1998) J. Immunol. 161:2772.
 

Alternate Names

GrzD

Entrez Gene IDs

14941 (Mouse)

Gene Symbol

GZMD

UniProt

Additional Granzyme D Products

Product Documents for Recombinant Mouse Granzyme D Protein, CF

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Recombinant Mouse Granzyme D Protein, CF

For research use only

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

÷
=