Product Specifications for Recombinant Mouse Granzyme D Protein, CF
Mouse myeloma cell line, NS0-derived mouse Granzyme D protein Ile21-Leu252 with a C-terminal 10-His tag
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain
<1.0 EU per 1 μg of the protein by the LAL method.
N-terminal sequence Analysis
Ile21 & His25
Predicted Molecular Mass
42 kDa, reducing conditions
Measured by its ability to cleave a peptide substrate, Succinyl-Phe-Leu-Phe-ThioBenzyl ester (Suc-FLF-SBzl), in the presence of 5,5’-Dithio-bis (2-nitrobenzoic acid) (DTNB). Edwards, K.M. et al. (1999) J. Biol. Chem. 274:30468. The specific activity is >50 pmol/min/µg, as measured under the described conditions.
Formulation, Preparation and Storage
What does CF mean?
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our
Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant
protein to be stored at a more dilute concentration.
The carrier free version does not contain BSA.
What formulation is right for me?
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or
as an ELISA standard.
In contrast, the carrier free protein is recommended for applications, in which the presence of BSA
Lyophilized from a 0.2 μm filtered solution in HEPES and NaCl.
Reconstitute at 100 μg/mL in sterile 25 mM HEPES and 200 mM NaCl, pH 7.5.
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
6 months from date of receipt, -20 to -70 °C as supplied.
3 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Granzyme D
Granzyme D is a member of the granzyme family of the serine proteases found specifically in the cytotoxic granules of cytotoxic T lymphocytes (CTL) and natural killer (NK) cells (1-3). Granzyme D’s functions are largely unknown. However, its selective expression in primary and functional hematopoietic stromal lines correlates with capacity of supporting growth of a pre-T lymphoma clone (4). Together with Granzymes E, F and G, it is regulated through pregnancy and by IL-2 and IL-15 in granulated metrial gland cells (5). The human or rat counterpart of mouse Granzyme D has not been found. Like other granzymes, mouse Granzyme D is not secreted as a zymogen but stored as a fully processed and activated enzyme in the cytoplasmic granules of CTL (1). It is synthesized as a 252 amino acid precursor with an 18 amino acid signal peptide and a 2 amino acid propeptide (1). The mature protein (residues 21-252) is expressed and purified.
Jenne, D.E. et al. (1988) Proc. Natl. Acad. Sci. USA 85:4814.
Kam, C.-M. et al. (2000) Biochim. Biophys. Acta 1477:307.
Smyth, M.J. et al. (1996) J. Leukoc. Biol. 60:555.
Yokoi, A. et al. (1999) Biochem. Biophys. Res. Comm. 264:768.
Allen, M.P. and M. Nilsen-Hamilton (1998) J. Immunol. 161:2772.
Entrez Gene IDs
CCP5, Ctla-5, Ctla5, Granzyme D, GrzD
Citations for Recombinant Mouse Granzyme D Protein, CF
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Product Documents for Recombinant Mouse Granzyme D Protein, CF
Which Brands are Currently Available on bio‑techne.com? R&D Systems, Tocris Bioscience and ProteinSimple branded products are available to purchase through bio‑techne.com. ProteinSimple branded instruments are available to quote. ACD branded products will be available on bio‑techne.com in the near future. Novus Biologicals branded products are not currently available on bio‑techne.com and can be found at novusbio.com.
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