Recombinant Human Nogo-A (aa 566-748) Fc Chimera Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 3515-NG

R&D Systems, part of Bio-Techne
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3515-NG-050
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Key Product Details

Source

CHO

Accession #

Q9NQC3

Structure / Form

Disulfide-linked homodimer

Conjugate

Unconjugated

Applications

Bioactivity

View all Nogo-A Proteins and Enzymes »

Product Specifications

Source

Chinese Hamster Ovary cell line, CHO-derived human Nogo-A protein
Human Nogo-A
(Val566 - Phe748) &
(Thr569 - Phe748)
Accession # Q9NQC3		 IEGRMD Human IgG1
(Pro100 - Lys330)
N-terminus C-terminus

Purity

>80%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Val566 & Thr569

Predicted Molecular Mass

46 kDa (monomer)

SDS-PAGE

70-90 kDa, reducing conditions

Activity

Measured by its ability to inhibit neurite outgrowth of dissociated E13 chick embryonic dorsal root ganglia (DRG) neurons.
Able to significantly inhibit neurite outgrowth when immobilized as a 3 µL droplet containing 200 ng on a nitrocellulose-coated microplate.

Reviewed Applications

Read 1 review rated 4 using 3515-NG in the following applications:

Formulation, Preparation and Storage

3515-NG
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution
Reconstitute at 100 μg/mL in sterile PBS.

Reconstitution Buffer Available:
Size / Price
Qty
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Nogo-A

Human Nogo-A (also reticulon-4) is a member of the reticulon family of transmembrane proteins. This family is characterized by the presence of a nonsignal sequence-containing N-terminus, a topologically conserved 200 amino acid (aa) C-terminus that contains two transmembrane domains with an ER-retention motif, and a punctate intracellular distribution within the ER that is reminescent of a reticulum (1 - 4). In human, Nogo exists in five isoforms (5 - 7). The full length human form (Nogo-A) is 1192 aa in length and contains a 1018 aa N-terminus, a 21 aa transmembrane segment, a 94 aa connecting “loop”, a second 21 aa transmembrane segment, and a 38 aa C-terminus. Three areas are of particular interest. One is a stretch of 66 aa within the 94 aa connecting loop. This segment is reported to bind to the GPI-linked Nogo receptor/p75 complex on axons and induce growth cone collapse (8 - 10). Two other areas in the N-terminus have also been discovered to have bioactivity (8, 11, 12). Based on rat, aa 57 - 184 in human (aa 59 - 172 in rat) should block fibroblast spreading, while aa 566 - 748 in human (aa 544 - 725 in rat) block neurite outgrowth and block fibroblast spreading (8, 12, 13). The exact topology of Nogo-A is unclear. The N- and C-termini may be extracellular with the “loop” region intracellular, or the situation could be reversed (13 - 15). Alternatively, the loop region and N-terminus may be on the same side of the membrane (3, 8). The four additional isoforms are shorter than Nogo-A (199 aa [Nogo-C], 373 aa [Nogo-B], 392 aa and 986 aa, respectively) (7). Although highly divergent, all contain the same C-terminal stretch, aa 1005 - 1192. Both Nogo-B and C are reported to complex with Nogo-A (16). Notably, Nogo-A is expressed in neurons, endothelial cells. oligodendrocytes, fibroblasts and myoblasts (12, 16 - 18). Human Nogo-A is 78% aa identical to mouse and rat Nogo-A overall, with 98% aa identity in the loop region and approximately 80% aa identity in the aa 566 - 748 segment.

References

  1. Oertle, T. et al. (2003) FASEB J. 17:1238.
  2. GrandPre, T. et al. (2000) Nature 403:439.
  3. Yan, R. et al. (2006) Cell. Mol. Life Sci. 63:877.
  4. Teng, F.Y.H. et al. (2004) J. Neurochem. 89:801.
  5. Chen, M.S. et al. (2000) Nature 403:434.
  6. Morris, N.J. et al. (1999) Biochim. Biophys. Acta 1450:68.
  7. GenBank Accession # Q9NQC3.
  8. Oertle, T. et al. (2003) J. Neurosci. 23:5393.
  9. Fournier, A.E. et al. (2001) Nature 409:341.
  10. Wang, K.C. et al. (2002) Nature 420:74.
  11. Prinjha, R. et al. (2000) Nature 403:384.
  12. Dodd, D.A. et al. (2005) J. Biol. Chem. 280:12494.
  13. Li, M. et al. (2004) Eur. J. Biochem. 271:3512.
  14. Huber, A.B. and M.E. Schwab (2000) Biol. Chem. 381:407.
  15. Ng, C.E.L. and B.L. Tang (2002) J. Neurosci. Res. 67:559.
  16. Dodd, D.A. et al. (2005) J. Biol. Chem. 280:12494.
  17. Wang, X. et al. (2002) J. Neurosci. 22:5505.
  18. Acevedo, L. et al. (2004) Nat. Med. 10:382.

Long Name

Reticulon 4A

Alternate Names

NI220, NogoA, RTN4, RTN4A

Entrez Gene IDs

57142 (Human); 68585 (Mouse); 83765 (Rat)

Gene Symbol

RTN4

UniProt

Q9NQC3

Additional Nogo-A Products

Product Documents for Recombinant Human Nogo-A (aa 566-748) Fc Chimera Protein, CF

Product Datasheets

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Product Specific Notices for Recombinant Human Nogo-A (aa 566-748) Fc Chimera Protein, CF

For research use only

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