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Recombinant Human Neuropilin-1 Fc Avi-tag Protein, CF

Bio-Techne includes R&D Systems | Catalog # AVI10455

Biotinylated
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AVI10455-050

Key Product Details

Learn more about Avi-tag Biotinylated Proteins

Source

HEK293

Accession #

Structure / Form

Disulfide-linked homodimer, biotinylated via Avi-tag

Conjugate

Unconjugated

Applications

Bioactivity

Product Specifications

Source

Human embryonic kidney cell, HEK293-derived human Neuropilin-1 protein
Human Neuropilin-1
(Phe22-Lys852)
Accession # ABY87548.1
IEGRMD Human IgG1
(Pro100-Lys330)
Avi-tag
N-terminus C-terminus

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Phe22

Predicted Molecular Mass

122 kDa

SDS-PAGE

120-140 kDa, under reducing conditions

Activity

Measured by its binding ability in a functional ELISA.
When Recombinant Human VEGF165  (Catalog # 293-VE) is immobilized at 25.0 ng/mL (100 µL/well), Biotinylated Recombinant Human Neuropilin‑1 Fc Chimera Avi-tag binds with an ED50 of 1.25-12.5 ng/mL.

The biotin to protein ratio is greater than 0.7 as determined by the HABA assay.

Scientific Data Images for Recombinant Human Neuropilin-1 Fc Avi-tag Protein, CF

Biotinylated Recombinant Human Neuropilin-1 Fc Chimera Avi-tag Protein Binding Activity.

When Recombinant Human VEGF 165 (239-VE) is immobilized at 25.0 ng/mL (100 μL/well), Biotinylated Recombinant Human Neuropilin-1 Fc Chimera Avi-tag (Catalog # AVI10455) binds with an ED50 of 1.25-12.5 ng/mL.

Biotinylated Recombinant Human Neuropilin-1 Fc Chimera Avi-tag Protein SDS-PAGE.

2 μg/lane of Biotinylated Recombinant Human Neuropilin-1 Fc Chimera Avi-tag Protein (Catalog # AVI10455) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 120-140 kDa and 240-280 kDa, respectively.

Formulation, Preparation and Storage

AVI10455
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Neuropilin-1

Neuropilin-1 (NRP1), also known as CD304, is a type I transmembrane glycoprotein involved in embryonic development, axon guidance and vasculature formation (1-4). The Neuropilin family is comprised of two isoforms, NRP1 and NRP2, that arose due to gene duplication and share 44% amino acid (aa) sequence identity (1). The extracellular domain (ECD) of Neuropilins are structurally similar containing two N-terminal CUB domains (termed a1/a2), two domains with homology to coagulation factors V and VIII (b1/b2) and a MAM (meprin) domain (5). The ECD of NRP1 shares 93% aa sequence identity with mouse and rat NRP1. Several C-terminally divergent splice variants of NRP1 and NRP2 lacking the MAM or transmembrane domain have been identified (5, 6). Neuropilins show partially overlapping expression in neuronal and endothelial cells during development (1, 2). They form homo- and hetero-oligomers via interaction of their MAM domains and share overlapping sets of ligands and functions (1, 7). Both Neuropilins act as co-receptors with Plexins and Semaphorins to help mediate axon repulsion (8, 9). NRP1 preferentially binds SEMA3A and PLXNA4, while NRP2 prefers binding to SEMA3F and PLXNA3 (8-10). NRP1 is capable of binding VEGF and forming signaling complexes with VEGFR2 to mediate cell migration (10, 11). There is evidence that NRP1 with VEGF plays a role in stemness of breast cancer cell by activating Wnt/b-catenin pathway (12). Additionally, NRP1 is expressed on dendritic cells and mediates DC‑induced T cell proliferation (13). NRP1 is a marker of CD4+ Treg cells and a population of CD8+ T-cells infiltrating solid tumors and immunotherapies blocking NRP1 synergize with anti-PD1 to enhance CD8+ proliferation and response (14). NRP1 has been shown to bind the S1 fragment of the SARS-CoV-2 spike protein and may potentially provide a therapeutic target for COVID-19 (15). Our Avi-tag Biotinylated NRP1 features biotinylation at a single site contained within the Avi-tag, a unique 15 amino acid peptide. Protein orientation will be uniform when bound to streptavidin-coated surface due to the precise control of biotinylation and the rest of the protein is unchanged so there is no interference in the protein's bioactivity.

References

  1. Bielenberg, D.R. et al. (2006) Exp. Cell Res. 312:584.
  2. Gu, C. et al. (2003) Dev. Cell 5:45.
  3. He, Z. and M. Tessier-Lavigne (1997) Cell 90:739.
  4. Soker, S. et al. (1998) Cell 92:735.
  5. Nakamura, F. and Goshima, Y. (2002) Adv Exp Med Biol. 515:55.
  6. Cackowski, F.C. et al. (2004) Genomics 84:82.
  7. Guo, H.F. and Vander Kooi, C.W. (2015) J Biol Chem. 290:29120.
  8. Yaron, A. et al. (2005) Neuron 45:513.
  9. Schwarz, Q. and Ruhrberg, C. (2010) Cell Adh Migr. 4:61
  10. Gu, C. et al. (2002) J. Biol. Chem. 277:18069
  11. Herzog, B. et al. (2011) Mol Biol Cell. 22:2766).
  12. Zhang, L. et al. (2017) Cell Physiol. Biochem. 44:1251.
  13. Tordjman, R. et al. (2002) Nat. Immunol. 3:477.
  14. Leclerc, M. et al. (2019) Nat Commun. 10:3345.
  15. Daly, J. et al. (2020) Science. 6518:861.

Alternate Names

BDCA-4, CD304, Neuropilin1, NRP1

Entrez Gene IDs

8829 (Human); 18186 (Mouse); 246331 (Rat); 100286859 (Porcine)

Gene Symbol

NRP1

UniProt

Additional Neuropilin-1 Products

Product Documents for Recombinant Human Neuropilin-1 Fc Avi-tag Protein, CF

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Recombinant Human Neuropilin-1 Fc Avi-tag Protein, CF

For research use only

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