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Recombinant Human Nectin-4 Fc Chimera Protein, CF

Catalog # 10682-N4 | R&D Systems, Inc. a Bio-Techne Brand
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10682-N4-050

Key Product Details

Source

HEK293

Accession #

Structure / Form

Disulfide-linked homodimer

Conjugate

Unconjugated

Applications

Bioactivity

Product Specifications

Source

Human embryonic kidney cell, HEK293-derived human Nectin-4 protein
Human Nectin-4
(Gly32-Ser349)
Accession # Q96NY8.1
IEGRMD Human IgG1
(Pro100-Lys330)
N-terminus C-terminus

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Gly32

Predicted Molecular Mass

61 kDa

SDS-PAGE

69-78 kDa, under reducing conditions

Activity

Measured by the ability of the immobilized protein to support the adhesion of NIH‑3T3 mouse embryonic fibroblast cells.
The ED50 for this effect is 0.35-4.20 µg/mL.

Scientific Data Images for Recombinant Human Nectin-4 Fc Chimera Protein, CF

Recombinant Human Nectin-4 Fc Chimera Protein Bioactivity Statement.

Recombinant Human Nectin-4 Fc Chimera (10682-N4) supports the adhesion of NIH-3T3 mouse embryonic fibroblast cells. The ED50 for this effect is 0.35-4.20 μg/mL.

Recombinant Human Nectin-4 Fc Chimera Protein SDS-PAGE.

2 μg/lane of Recombinant Human Nectin-4 Fc Chimera Protein (Catalog # 10682-N4) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 69-78 kDa and 138-156 kDa, respectively.

Formulation, Preparation and Storage

10682-N4
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Nectin-4

Nectin-4 (gene name PVRL4, poliovirus receptor-like 4) is a 66 kDa type I transmembrane glycoprotein belonging to the Nectin family of Ig superfamily proteins (1). The Latin word necto means “to connect”, indicating the role of nectins in Ca2+‑independent cell-cell adhesion (2). Nectin-4 forms homodimers in cis, followed by interactions in trans with Nectin-1 or -4 (1-3). Human Nectin-4 mRNA is normally expressed in the placenta, especially in endothelial cells, while in the mouse it is found in the embryo, lung, testis and brain (1, 4, 5). Human Nectin-4 cDNA encodes 510 amino acids (aa), including a 31 aa signal sequence, a 318 aa extracellular domain (ECD), a 21 aa transmembrane segment (TM), and a 140 aa cytoplasmic region. Nectin ECDs contain three Ig-like domains: an N‑terminal V-type that mediates ligand binding, and two C2-type (1, 3). One Nectin‑4 isoform lacks aa 412-436 in the cytoplasmic domain (1). In many human ductal breast or non-small cell lung carcinomas, Nectin‑4 is upregulated and a soluble 43 kDa form is found in the plasma (4-6). This form is generated from the membrane protein via the action of TACE/ADAM-17 (6). The extracellular domain of human Nectin-4 shares 91%, 92%, 93%, 91% and 90% amino acid sequence homology with the corresponding regions of mouse, rat, canine, porcine and bovine Nectin-4, respectively. In forming adherens junctions, trans interactions of Nectin-4 initiate cell-cell interactions and recruit intracellular cadherins through afadin and other junctional proteins (1, 2). These interactions organize the actin cytoskeleton, strengthen attachment to basement membrane and promote further cell-cell connections (2, 7). In humans, mutation of Nectin-4 has been correlated with ectodermal dysplasia-syndactyly syndrome, indicating a role for Nectin-4 in human development (7).

References

  1. Reymond, N. et al. (2001) J. Biol. Chem. 276:43205.
  2. Takai, Y. et al. (2008) Nat. Rev. Mol. Cell Biol. 9:603.
  3. Fabre, S. et al. (2002) J. Biol. Chem. 277:27006.
  4. Fabre-Lafay, S. et al. (2007) BMC Cancer 7:73.
  5. Takano, A. et al. (2009) Cancer Res. 69:6694.
  6. Fabre-Lafay, S. et al. (2005) J. Biol. Chem. 280:19543.
  7. Brancati, F. et al. (2010) Am. J. Hum. Genet. 87:265.
  8. Challita-Eid, P.M. et al. (2016) Cancer Res. 76:3003.

Long Name

Poliovirus Receptor Related 4

Alternate Names

LNIR, Nectin4, PRR4, PVRL4

Entrez Gene IDs

81607 (Human); 71740 (Mouse); 498281 (Rat); 102127936 (Cynomolgus Monkey)

Gene Symbol

NECTIN4

UniProt

Product Documents for Recombinant Human Nectin-4 Fc Chimera Protein, CF

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Recombinant Human Nectin-4 Fc Chimera Protein, CF

For research use only

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