Skip to main content

Novus Biologicals products are now on bio-techne.com

Better as one

Recombinant Human Fetuin B Protein, CF

Bio-Techne includes R&D Systems | Catalog # 1725-PI

Catalog #
Availability
Size / Price
Qty
Loading...
1725-PI-050

Key Product Details

Source

NS0

Accession #

Conjugate

Unconjugated

Applications

Inhibition Activity

Product Specifications

Source

Mouse myeloma cell line, NS0-derived human Fetuin B protein
Met19-Pro382, with a C-terminal 10-His tag

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<1.0 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Met19

Predicted Molecular Mass

42 kDa

SDS-PAGE

54 kDa and 58 kDa, reducing conditions

Activity

Measured by its ability to inhibit active Cathepsin V cleavage of a fluorogenic peptide substrate Z-LR-AMC (Catalog # ES008).
The IC50 value is <150 nM, as measured under the described conditions.

Formulation, Preparation and Storage

1725-PI
Formulation Supplied as a 0.2 μm filtered solution in Tris and NaCl.
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -20 to -70 °C as supplied.
  • 3 months, -20 to -70 °C under sterile conditions after opening.

Background: Fetuin B

Fetuins are members of the cystatin superfamily of cysteine protease inhibitors (1-3). Additional members of this superfamily are kininogen and histidine-rich glycoprotein. Fetuin A and B are two known members of the fetuin family. Hepatocytes are believed to be the principal cellular source, but other cell types also express it (4, 5). Fetuin A, also known as alpha2-Heremans-Schmid glycoprotein, is an inhibitor of basic calcium phosphate precipitation and a negative acute-phase protein (6, 7). Normal circulating levels of Fetuin A in adults (300-600 μg/mL) fall significantly (30-50%) during injury and infection (7). Fetuin B is a newer member whose function is not fully characterized (1, 2). Fetuin A and B display similarities and differences in their characteristics. Fetuin B exhibits reduction of calcification, while both mRNA levels were down‑regulated during the acute phase in inflammation-induced rats (4). However, they share only 20% amino acid sequence identity (2). The amounts of Fetuin B in human serum, unlike Fetuin A, vary with gender and are higher in females than in males (4).

References

  1. Oliver, E., et al. (1999) Genomics. 57:352.
  2. Oliver, E., et al. (2000) Biochem. J. 350:589.
  3. Kellemann, J. et al., 1989, J. Biol. Chem. 264:14121.
  4. Denecke, B. et al. (2003) Biochem. J. 376:135.
  5. Schäfer, C., et al. (2003) J. Clin. Invest. 112:357.
  6. Dziegielewska, K. M., et al. (1996) Histochem. Cell Biol. 106:319.
  7. Gangneux, C. et al. (2003) Nucleic Acids Res. 31:5957.

Alternate Names

FETUB

Entrez Gene IDs

26998 (Human)

Gene Symbol

FETUB

UniProt

Additional Fetuin B Products

Product Documents for Recombinant Human Fetuin B Protein, CF

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Recombinant Human Fetuin B Protein, CF

For research use only