Recombinant Human Dectin-2/CLEC6A Protein, CF

Name: Recombinant Human Dectin-2/CLEC6A Protein, CF
Brand: R&D Systems
SKU: 3114-DC

R&D Systems, Inc. a Bio-Techne Brand

Catalog # 3114-DC

Citations (4)

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Key Product Details

Source:	NS0
Accession #:	Q6EIG7
Applications:	Binding Activity

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Product Specifications for Recombinant Human Dectin-2/CLEC6A Protein, CF

Source	Mouse myeloma cell line, NS0-derived human Dectin-2/CLEC6A protein Thr46-Leu209, with an N-terminal 6-His tag
Purity	>90%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin level	<0.01 EU per 1 μg of the protein by the LAL method.
N-terminal sequence Analysis	His
Predicted Molecular Mass	19.7 kDa
SDS-PAGE	23 kDa, reducing conditions
Activity	Measured by its ability to bind biotinylated alpha -D-Mannose-PAA in a functional ELISA.

Formulation, Preparation and Storage

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

3114-DC

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 100 μg/mL in sterile PBS. Reconstitution Buffer Available: Size / Price Qty Loading... RB01:
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

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The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Dectin-2/CLEC6A

Dectin-2, also known as CLEC6A, CLECSF10, and NKCL, belongs to the C-type lectin family of transmembrane immune regulatory glycoproteins. Dectin-2, plus CLEC4A-E constitute a subgroup of molecules that exhibit approximately 40% amino acid (aa) sequence identity in their extracellular domains (ECD), and have a conserved cysteine spacing in their carbohydrate recognition domains (CRD) (1, 2). Mature human Dectin-2 is a type II transmembrane protein with a short cytoplasmic tail, a transmembrane segment, and a 168 aa ECD with a stalk region and one CRD (3, 4). Within the ECD, human Dectin-2 shares 71% and 75% aa sequence identity with bovine and mouse Dectin-2, respectively. An alternately spliced beta isoform has a deletion of portions of the transmembrane and cytoplasmic regions (5). Full length Dectin-2 is a 27 kDa molecule that is expressed on monocytes, tissue macrophages, and activated CD4⁺ T cells (4 - 6). The CRD of Dectin-2 contains an EPN motif which is characteristic of calcium-dependent mannose-binding lectins. Dectin-2 selectively interacts with high mannose structures in the Man₉GlcNAc₂ configuration (7). It mediates the recognition of a variety of microorganisms, particularly the filamentous forms of yeast and fungii (7, 8). The short cytoplasmic tail does not contain signaling motifs but mediates association with the ITAM-containing Fc receptor gamma subunit on macrophages (8). Ligation of Dectin-2 induces tyrosine phosphorylation of the gamma subunit, activation of NF kappa B, and enhanced release of TNF-alpha and IL-1ra (8). Macrophage Dectin-2 is upregulated in vivo by inflammatory stimuli and UV-B irradiation (5, 6, 9). Dectin-2 is known to participate in UV-induced immunosuppression by interacting with CD4⁺CD25⁺ regulatory T cells, which then induce dendritic cells to release IL-4, IL-10, and TGF-beta (10).

Item	Value
References	Kanazawa, N. (2007) J. Dermatol. Sci. 45:77. Kanazawa, N. et al. (2004) Immunobiology 209:179. Flornes, L.M. et al. (2004) Immunogenetics 56:506. Kanazawa, N. et al. (2004) J. Invest. Dermatol. 122:1522. Gavino, A.C. et al. (2005) Exp. Dermatol. 14:281. Taylor, P.R. et al. (2005) Eur. J. Immunol. 35:2163. McGreal, E.P. et al. (2006) Glycobiology 16:422. Sato, K. et al. (2006) J. Biol. Chem. 281:38854. Bonkobara, M. et al. (2005) Photochem. Photobiol. 81:944. Aragane, Y. et al. (2003) J. Immunol. 171:3801.
Entrez Gene IDs	93978 (Human); 56620 (Mouse)
Alternate Names	C-type (calcium dependent, carbohydrate-recognition domain) lectin, superfamilymember 10, C-type lectin domain family 6 member A, C-type lectin domain family 6, member A, C-type lectin superfamily member 10, CLEC4N, CLEC6A, CLECSF10, DC-associated C-type lectin 2, Dectin-2, Dectin2, Dendritic cell-associated C-type lectin 2, dectin 2

Citations for Recombinant Human Dectin-2/CLEC6A Protein, CF (4)

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Citations are publications that use Bio-Techne products. Selected citations for Recombinant Human Dectin-2/CLEC6A Protein, CF include:

Species: Human
Sample Types: Protein
Applications: Bioassay

M Bruno et al. (2020), Transcriptional and functional insights into the host immune response against the emerging fungal pathogen Candida auris Nat Microbiol, 0(0).
PMID: 32839538
Species: Human
Sample Types: Protein
Applications: Bioassay

M Bruno et al. (2020), Transcriptional and functional insights into the host immune response against the emerging fungal pathogen Candida auris Nat Microbiol, 0(0).
PMID: 32839538
Species: Human
Sample Types: Recombinant Protein
Applications: Force Microscopy

C Leclaire et al. (2018), Molecular basis for intestinal mucin recognition by galectin-3 and C-type lectins FASEB J., 0(0):fj201700619R.
PMID: 29401627
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

KP Bene et al. (2017), Lactobacillus reuteri Surface Mucus Adhesins Upregulate Inflammatory Responses Through Interactions With Innate C-Type Lectin Receptors Front Microbiol, 8(0):321.
PMID: 28326063

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Showing 1 - 4 of 4 citations Showing All

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Recombinant Human Dectin-2/CLEC6A Protein, CF

R&D Systems, Inc. a Bio-Techne Brand

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