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Recombinant Human CXCL6/GCP-2 Protein, CF
Catalog # 333-GC/CF | R&D Systems, Inc. a Bio-Techne Brand
Key Product Details
Product Specifications
Source
E. coli-derived human CXCL6/GCP-2 protein
Val40-Asn114
Val40-Asn114
Purity
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
N-terminal Sequence Analysis
Val40
Predicted Molecular Mass
8 kDa
Activity
Measured by its ability to chemoattract BaF3 mouse pro‑B cells transfected with human CXCR2.
The ED50 for this effect is 3‑15 ng/mL.
Measured by its ability to induce myeloperoxidase release from cytochalasin B-treated human neutrophils. Schröder, J.M. et al. (1987) J. Immunol. 139:3474.
The ED50 for this effect is 0.5‑1.5 µg/mL.
The ED50 for this effect is 3‑15 ng/mL.
Measured by its ability to induce myeloperoxidase release from cytochalasin B-treated human neutrophils. Schröder, J.M. et al. (1987) J. Immunol. 139:3474.
The ED50 for this effect is 0.5‑1.5 µg/mL.
Formulation, Preparation and Storage
333-GC/CF
| Formulation | Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA. |
| Reconstitution | Reconstitute at 100 μg/mL in sterile PBS. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Background: CXCL6/GCP-2
GCP-2 (granulocyte chemotactic protein-2) also known as CXCL6, is a CXC chemokine initially isolated as a neutrophil chemoattractant from the MG-63 osteosarcoma cell line. Among human CXC chemokines, GCP-2 is most closely related to ENA-78 (78% amino acid (aa) sequence identity in the mature peptide region and 86% identity in the signal sequence). The structure and sequence of the genes for human GCP-2 and ENA-78 also exhibit close similarity suggesting the two genes may have originated from a gene duplication. LIX (LPS-induced CXC chemokine) was initially cloned as a gene induced by LPS in mouse fibroblasts. The predicted LIX protein sequence is identical to a previously purified mouse protein designated mouse GCP-2 based on its amino sequence similarity (60% sequence identity) to human GCP-2. Mouse GCP-2/LIX is also 54% identical with human ENA-78 at the amino acid sequence level.
Human GCP-2 cDNA encodes a propeptide of 114 amino acid residues with a predicted 37 aa residue signal peptide and 77 aa residue mature protein. Several forms of natural GCP-2 have been isolated from MG-63 conditioned media, indicating that GCP-2 undergoes limited processing at both the N- and C-termini. Human GCP-2 is a primary response gene whose induction by cytokines is attenuated by dexamethasone.
Human GCP-2 and mouse GCP-2/LIX have been shown to chemoattract and activate neutrophils, but not eosinophils and monocytes. It is likely that GCP-2 activities are mediated via the human or mouse CXCR2.
Product Documents for Recombinant Human CXCL6/GCP-2 Protein, CF
Product Specific Notices for Recombinant Human CXCL6/GCP-2 Protein, CF
For research use only
Citations for Recombinant Human CXCL6/GCP-2 Protein, CF (5)
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Citations are publications that use Bio-Techne products. Selected citations for Recombinant Human CXCL6/GCP-2 Protein, CF include:
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Species: Human
Sample Types: Whole Cells
Applications: BioassayJC Ma et al. (2017-07-28), Fibroblast-derived CXCL12/SDF-1? promotes CXCL6 secretion and co-operatively enhances metastatic potential through the PI3K/Akt/mTOR pathway in colon cancer World J. Gastroenterol., 2017-023(28):5167-5178.
PMID: 28811711 -
Species: Human
Sample Types: Whole Cells
Applications: Bioassay(2012-07-31), Staphylococcus aureus Staphopain A inhibits CXCR2-dependent neutrophil activation and chemotaxis. EMBO J., 2012-031(17):3607-19.
PMID: 22850671 -
Species: Human
Sample Types: Whole Cells
Applications: BioassayWilson TR et al. (2012-07-26), Widespread potential for growth-factor-driven resistance to anticancer kinase inhibitors. Nature, 2012-0487(7408):505-9.
PMID: 22763448 -
Species: Human
Sample Types: Whole Cells
Applications: BioassayBegley LA et al. (2008-06-24), The inflammatory microenvironment of the aging prostate facilitates cellular proliferation and hypertrophy. Cytokine, 2008-043(2):194-9.
PMID: 18572414 -
Species: Human
Sample Types: Whole Cells
Applications: BioassayNguyen AN et al. (2006-04-04), Normalizing the bone marrow microenvironment with p38 inhibitor reduces multiple myeloma cell proliferation and adhesion and suppresses osteoclast formation. Exp. Cell Res., 2006-0312(10):1909-23.
PMID: 16600214
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