Recombinant Human Coagulation Factor VII Protein, CF

Catalog # 2338-SE | R&D Systems, Inc. a Bio-Techne Brand
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2338-SE-010
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Key Product Details

Accession #

NP_062562

Source

NS0

Structure / Form

Mature form

Conjugate

Unconjugated

Applications

Enzyme Activity

Product Specifications

Source

Mouse myeloma cell line, NS0-derived human Coagulation Factor VII protein
Ala39-Pro444, with a C-terminal 10-His tag

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<1.0 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Ala39

Predicted Molecular Mass

46 kDa

SDS-PAGE

60 kDa, reducing conditions

Activity

Measured by its ability to cleave the fluorogenic peptide substrate Boc-VPR-AMC (Catalog # ES011).
The specific activity is >50 pmol/min/µg, as measured under the described conditions.

Formulation, Preparation and Storage

2338-SE
Formulation Supplied as a 0.2 μm filtered solution in Sodium Acetate and NaCl.
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -20 to -70 °C as supplied.
  • 3 months, -20 to -70 °C under sterile conditions after opening.

Background: Coagulation Factor VII

Coagulation Factors VII and VIIa refer to the pro and active forms of the same protease, respectively (1). Factor VII is synthesized in the liver and circulates in the plasma where it binds to tissue factor (TF), an integral membrane protein found in a variety of cell types. Upon binding of TF, Factor VII is rapidly converted into VIIa. The resulting 1:1 complex of VIIa and TF initiates the coagulation pathway and has also important coagulation-independent functions such as angiognesis (2). The cleavage and activation of Coagulation Factors VII, IX and X by VIIa:TF is phospholipid-dependent whereas the cleavage of small peptide substrates is not (1). The predominant splicing variant of Factor VII in normal liver corresponds to the 444 amino acid precursor (3, 4). After a signal peptide (residues 1 to 38), the mature chain can be further processed into the light chain (residues 39 to 190) and the heavy chain (residues 191 to 444). The purified Recombinant Human Factor VII corresponds to the mature chain, which can be processed and activated by treatment with thermolysin and binding with Recombinant Human Coagulation Factor III/Tissue Factor (Catalog # http://www.rndsystems.com/product_results.aspx?k=2339-PA">2339-PA) under the conditions described above.

References

  1. Morrissey, J.H. (2004) in Handbook of Proteolytic Enzymes, Barrett, A.J. et al. eds. p. 1659. 
  2. Versteeg, H.H. et al. (2003) Carcinogenesis 24:1009.
  3. Hagen, F.S, et al. (1986) Proc. Natl. Acad. Sci. USA 83:2412.
  4. O’Hara, P.J. et al. (1987) Proc. Natl. Acad. Sci. USA 84:5158.

Long Name

Coagulation Factor VII (Serum Prothrombin Conversion Accelerator)

Alternate Names

F7

Entrez Gene IDs

2155 (Human); 14068 (Mouse)

Gene Symbol

F7

UniProt

NP_062562

Product Documents for Recombinant Human Coagulation Factor VII Protein, CF

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Recombinant Human Coagulation Factor VII Protein, CF

For research use only

Citations for Recombinant Human Coagulation Factor VII Protein, CF (2)

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    Name: Anonymous
    Application: Enzymatic activity in vitro
    Verified Customer | Posted 08/03/2016

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FAQs for Recombinant Human Coagulation Factor VII Protein, CF

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  • Q: Is the addition of Coagulation Factor III/Tissue Factor necessary for the activity of Coagulation Factor VII?

    A: Our testing has shown minimal detectable kinetic activity of Coagulation Factor VII following cleavage with Thermolysin in the absence of Tissue Factor.

    Upon binding with Tissue Factor (TF), Coagulation Factor VII is rapidly converted into Coagulation Factor VIIa. The resulting 1:1 complex of VIIa and TF initiates the coagulation pathway. This complex also has important coagulation-independent functions such as angiogenesis.

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