Human embryonic kidney cell, HEK293-derived human CNTFR/CNTF Complex protein
Human CNTFR (Gln23-Ser342) Accession # P26992
Human CNTF (Met1-Met200) Accession # P26441
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
<0.10 EU per 1 μg of the protein by the LAL method.
N-terminal sequence Analysis
Gln23 inferred from deblocking revealing Arg24
Predicted Molecular Mass
74-85 kDa, under reducing conditions
Measured in a cell proliferation assay using TF‑1 human erythroleukemic cells. Kitamura, T. et al. (1989) J. Cell Physiol. 140:323. The ED50 for this effect is 0.25-1.5 ng/mL.
Recombinant Human CNTFR/CNTF Complex Protein, CF Scientific Data Examples
Recombinant Human CNTFR/CNTF Complex Protein Bioactivity
Recombinant Human CNTFR/CNTF Complex (Catalog # 10283-CC) stimulates proliferation of the TF-1 human erythroleukemic cell line. The ED50 for this effect is 0.25-1.5 ng/mL. The Recombinant Human CNTFR/CNTF Complex has up to 100-fold greater activity than Recombinant CNTF alone (Catalog # 257-NT).
Recombinant Human CNTFR/CNTF Complex Protein SDS-PAGE
2 μg/lane of Recombinant Human CNTFR/CNTF Complex was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 74-85 kDa.
Formulation, Preparation and Storage
What does CF mean?
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our
Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant
protein to be stored at a more dilute concentration.
The carrier free version does not contain BSA.
What formulation is right for me?
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or
as an ELISA standard.
In contrast, the carrier free protein is recommended for applications, in which the presence of BSA
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitute at 200 μg/mL in PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
3 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CNTFR/CNTF Complex
Human CNTF (ciliary neurotrophic factor) encodes a 200 amino acid polypeptide that lacks a signal sequence. It shares 83% and 84% sequence identity with the mouse and rat homologs, respectively. CNTF is known to be expressed by Schwann cells, astrocytes and T cells (1). CNTF is structurally related to IL-6, IL-11, LIF and OSM. All of these four helix bundle cytokines share gp130 as a signal-transducing subunit in their receptor complexes (2, 3). Engagement of CNTF with CNTFR alpha, LIFR, and gp130 on CD4 T cells has been shown to increase the number of IFN-gamma producing differentiated cells. IFN-gamma being a mediator of IFN-beta suggests CNTF's role in therapeutic effects of IFN-beta in multiple sclerosis (1, 4). CNTFR alpha contains a 22 amino acid signal peptide, 320 amino acids mature domain and a 30 amino acids propeptide. The mature human CNTFR alpha contains one Ig-like C2-type domain and two Fibronectin type III domains. CNTFR alpha differs from other cytokine receptors in that it lacks transmembrane and cytoplasmic domains and is anchored to cell membranes by a glycosylphosphatidylinositol (GPI) linkage (5). Mature human CNTFR alpha shares 98% and 96% sequence identity with its mouse and rat homologs in the mature domain, respectively. Similar to other GPI-linked proteins, soluble CNTFR alpha (CNTF sR alpha ) can be released from the cell surface by phosphatidylinositol-specific phospholipase C. CNTF sR alpha can be released from skeletal muscle in response to peripheral nerve injury and high concentrations of CNTF sR alpha have also been detected in human cerebrospinal fluid (4). CNTF sR alpha binds CNTF in solution and the complex can act on cells that express only LIF R beta and gp130 but not CNTFR alpha (4, 6).
Tormo, A.J. et al. (2012) Cytokine 60:65.
Sleeman, M.W. et al. (2000). Pharma Acta Helv. 74:265.
Bravo J. et al. (2000) EMBO J. 19:2399
Pasquin, S. et al. (2015) Cytokine Growth Factor 26:507.
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