Recombinant Human CCL14a/HCC-1 Protein, CF

Name: Recombinant Human CCL14a/HCC-1 Protein, CF
Brand: R&D Systems
SKU: 324-HC/CF

R&D Systems, Inc. a Bio-Techne Brand

Catalog # 324-HC/CF

Citations (3)

Product Datasheet / COA / SDS

Discontinued Product

324-HC/CF has been discontinued. View all CCL14a/HCC-1 products.

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Key Product Details

Source:	E. coli
Accession #:	Q16627
Applications:	Bioactivity

View Full Technical Details for Recombinant Human CCL14a/HCC-1 Protein, CF

Product Specifications for Recombinant Human CCL14a/HCC-1 Protein, CF

Source	E. coli-derived human CCL14a/HCC-1 protein Thr20-Asn93
Purity	>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin level	<0.01 EU per 1 μg of the protein by the LAL method.
N-terminal sequence Analysis	Thr20
Predicted Molecular Mass	8.7 kDa
Activity	Measured by its ability to chemoattract BaF3 mouse pro‑B cells transfected with human CCR1. The ED₅₀ for this effect is 0.2‑0.8 µg/mL.

Formulation, Preparation and Storage

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

324-HC/CF

Carrier Free

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 100 μg/mL in sterile PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: CCL14a/HCC-1

HCC-1 (Hemofiltrate CC Chemokine-1) was originally isolated from the hemofiltrate of human patients with chronic renal failure (1). It belongs to the CC chemokine superfamily and has been designated CCL14a. HCC-1/CCL14a cDNA encodes a 93 amino acid (aa) residue precursor with a 19 aa signal peptide that is cleaved to form the 74 aa secreted protein (aa 20 - 93). By alternative splicing, a second longer isoform named HCC-3/CCL14b, which includes sequences from exon 3, also exists (2). HCC-1/CCL14a is expressed constitutively in various normal tissues including spleen, liver, muscle, gut and bone marrow. It circulates at nanomolar concentrations in human plasma. Different post-translationally modified HCC-1/CCL14a, including O-glycosylated and N-terminally truncated variants of HCC-1/CCL14a, have been identified (3, 4). Whereas the 74 aa peptide is a weak CCR1 agonist, the proteolytically processed, truncated HCC-1/CCL14a (aa 28 - 93) is a highly potent agonist of CCR1, CCR5 and to a lesser extent, CCR3. HCC-1/CCL14a (aa 28 - 93) promotes chemotaxis of T lymphocytes, monocytes and eosinophils, and inhibits infection of M-tropic human immunodeficiency virus type 1. Activation of the HCC-1/CCL14a precursor to active peptide is mediated by the urokinase type plasminogen activator or plasmin (5).

Item	Value
References	Schulz-Knappe, P. et al. (1996) J. Exp. Med. 183:295. Forssmanns, U. et al. (2001) J. Leukocyte Biology 70:357. Richter, R. et al. (2000) Biochemistry 39:10799. Munch, J. et al. (2002) Antimicrob. Agents Chemother. 46:982. Vakili, J. et al. (2001) J. Immunol. 167:3406.
Entrez Gene IDs	6358 (Human)
Alternate Names	CCL14, CCL14a, HCC-1, HCC1

Citations for Recombinant Human CCL14a/HCC-1 Protein, CF (3)

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Citations are publications that use Bio-Techne products. Selected citations for Recombinant Human CCL14a/HCC-1 Protein, CF include:

Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Wilson TR et al. (2012), Widespread potential for growth-factor-driven resistance to anticancer kinase inhibitors. Nature, 487(7408):505-9.
PMID: 22763448
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Huber TB et al. (2002), Expression of functional CCR and CXCR chemokine receptors in podocytes. J. Immunol., 168(12):6244-52.
PMID: 12055238
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Cashman J et al. (2002), Stromal-derived factor 1 inhibits the cycling of very primitive human hematopoietic cells in vitro and in NOD/SCID mice. Blood, 99(3):792-9.
PMID: 11806978

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Product Documents for Recombinant Human CCL14a/HCC-1 Protein, CF

Product Datasheets

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Recombinant Human CCL14a/HCC-1 Protein, CF

R&D Systems, Inc. a Bio-Techne Brand

Key Product Details

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Product Specifications for Recombinant Human CCL14a/HCC-1 Protein, CF

Formulation, Preparation and Storage

Carrier Free

Carrier Free

Reconstitution Calculator

Background: CCL14a/HCC-1

Citations for Recombinant Human CCL14a/HCC-1 Protein, CF (3)

Product Documents for Recombinant Human CCL14a/HCC-1 Protein, CF

Product Datasheets

COA

Certificate of Analysis

SDS

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