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Recombinant Human CCL13/MCP-4 Protein, CF

Catalog # 327-P4/CF | R&D Systems, Inc. a Bio-Techne Brand
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Key Product Details

Accession #


E. coli





Product Specifications


E. coli-derived human CCL13/MCP-4 protein


>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis


Predicted Molecular Mass

8.6 kDa


Measured by its ability to chemoattract 2-day cultured human monocytes. Matsushima, K. et al. (1989) J. Exp. Med. 169:1485.
The ED50 for this effect is 0.02-0.06 µg/mL.

Measured by its ability to chemoattract BaF3 mouse pro‑B cells transfected with human CCR2A.
The ED50 for this effect is 0.5-2 µg/mL.

Formulation, Preparation and Storage

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 100 μg/mL in sterile deionized water.  
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CCL13/MCP-4

Human CCL13 is a CC chemokine recently cloned from a human fetal and a human heart cDNA library. Human CCL13 cDNA encodes a 98 amino acid residue precursor protein with a 23 amino acid residue hydrophobic signal peptide that is cleaved to yield an 8 kDa, 75 aa mature CCL13. Mature CCL13 lacks any potential N-glycosylation sites and shares a pyroglutamate proline motif with other human MCP proteins. Human CCL13 is most homologous to MCP-1, 3 and Eotaxin, exhibiting approximately 65 - 66% amino acid sequence identity. CCL13 mRNA is expressed by a number of activated cell types, including endothelial cells, macrophages, bronchial epithelium and type II alveolar cells, and perhaps lymphocytes. CCL13 is a chemoattractant for monocytes and eosinophils, and activates basophils. In addition, it has been reported to be chemotactic for CD4+ and CD8+ T cells, with an activity almost equivalent to that of MCP-3. The bioactivities of CCL13 is most likely mediated by the CC chemokine receptors CCR-2 and CCR-3, both of which have been shown to bind CCL13.


  1. Garcia-Zepeda, E.A. et al. (1996) J. Immunol. 157:5613.
  2. Uguccioni, M. et al. (1996) J. Exp. Med. 183:2379.
  3. Godisha, R. et al. (1997) J. Leukoc, Biol. 61:353.
  4. Stellato, C. et al. (1997) J. Clin. Invest. 99:926.
  5. Heath, H. et al. (1997) J. Clin. Invest. 99:178.
  6. Charo, I.F. et al. (1994) Proc. Natl. Acad. Sci. USA 91:2752.
  7. Daugherty, B.L. et al. (1996) J. Exp. Med. 183:2349.
  8. Combadiere, C. et al. (1995) J. Biol. Chem. 270:16491.

Alternate Names


Entrez Gene IDs

6357 (Human)

Gene Symbol



Product Documents for Recombinant Human CCL13/MCP-4 Protein, CF

Certificate of Analysis

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Product Specific Notices for Recombinant Human CCL13/MCP-4 Protein, CF

For research use only

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