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Recombinant Human CCL13/MCP-4 Protein, CF
Catalog # 327-P4/CF | R&D Systems, Inc. a Bio-Techne Brand
Key Product Details
Product Specifications
Source
E. coli-derived human CCL13/MCP-4 protein
Gln24-Thr98
Gln24-Thr98
Purity
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
N-terminal Sequence Analysis
Gln24
Predicted Molecular Mass
8.6 kDa
Activity
Measured by its ability to chemoattract 2-day cultured human monocytes. Matsushima, K. et al. (1989) J. Exp. Med. 169:1485.
The ED50 for this effect is 0.02-0.06 µg/mL.
Measured by its ability to chemoattract BaF3 mouse pro‑B cells transfected with human CCR2A.
The ED50 for this effect is 0.5-2 µg/mL.
The ED50 for this effect is 0.02-0.06 µg/mL.
Measured by its ability to chemoattract BaF3 mouse pro‑B cells transfected with human CCR2A.
The ED50 for this effect is 0.5-2 µg/mL.
Formulation, Preparation and Storage
327-P4/CF
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS. |
| Reconstitution | Reconstitute at 100 μg/mL in sterile deionized water. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Background: CCL13/MCP-4
Human CCL13 is a CC chemokine recently cloned from a human fetal and a human heart cDNA library. Human CCL13 cDNA encodes a 98 amino acid residue precursor protein with a 23 amino acid residue hydrophobic signal peptide that is cleaved to yield an 8 kDa, 75 aa mature CCL13. Mature CCL13 lacks any potential N-glycosylation sites and shares a pyroglutamate proline motif with other human MCP proteins. Human CCL13 is most homologous to MCP-1, 3 and Eotaxin, exhibiting approximately 65 - 66% amino acid sequence identity. CCL13 mRNA is expressed by a number of activated cell types, including endothelial cells, macrophages, bronchial epithelium and type II alveolar cells, and perhaps lymphocytes. CCL13 is a chemoattractant for monocytes and eosinophils, and activates basophils. In addition, it has been reported to be chemotactic for CD4+ and CD8+ T cells, with an activity almost equivalent to that of MCP-3. The bioactivities of CCL13 is most likely mediated by the CC chemokine receptors CCR-2 and CCR-3, both of which have been shown to bind CCL13.
References
- Garcia-Zepeda, E.A. et al. (1996) J. Immunol. 157:5613.
- Uguccioni, M. et al. (1996) J. Exp. Med. 183:2379.
- Godisha, R. et al. (1997) J. Leukoc, Biol. 61:353.
- Stellato, C. et al. (1997) J. Clin. Invest. 99:926.
- Heath, H. et al. (1997) J. Clin. Invest. 99:178.
- Charo, I.F. et al. (1994) Proc. Natl. Acad. Sci. USA 91:2752.
- Daugherty, B.L. et al. (1996) J. Exp. Med. 183:2349.
- Combadiere, C. et al. (1995) J. Biol. Chem. 270:16491.
Alternate Names
MCP-4, MCP4
Entrez Gene IDs
6357 (Human)
Gene Symbol
CCL13
UniProt
Product Documents for Recombinant Human CCL13/MCP-4 Protein, CF
Product Specific Notices for Recombinant Human CCL13/MCP-4 Protein, CF
For research use only
Citations for Recombinant Human CCL13/MCP-4 Protein, CF (5)
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Citations are publications that use Bio-Techne products. Selected citations for Recombinant Human CCL13/MCP-4 Protein, CF include:
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Species: Human
Sample Types: Whole Cells
Applications: BioassayWilson TR et al. (2012-07-26), Widespread potential for growth-factor-driven resistance to anticancer kinase inhibitors. Nature, 2012-0487(7408):505-9.
PMID: 22763448 -
Sample Types: N/A
Applications: ELISA (Standard)Gounni Abdelilah S et al. (2006-06-16), Increased expression of Th2-associated chemokines in bullous pemphigoid disease. Role of eosinophils in the production and release of these chemokines. Clin. Immunol., 2006-0120(2):220-31.
PMID: 16782408 -
Species: Human
Sample Types: Cell Culture Supernates
Applications: BioassayYamamoto H et al. (2005-02-01), CC chemokines and transmigration of eosinophils in the presence of vascular cell adhesion molecule 1. Ann. Allergy Asthma Immunol., 2005-094(2):292-300.
PMID: 15765748 -
Species: Human
Sample Types: Whole Cells
Applications: BioassayKohrgruber N et al. (2004-12-01), Plasmacytoid dendritic cell recruitment by immobilized CXCR3 ligands. J. Immunol., 2004-1173(11):6592-602.
PMID: 15557149 -
Species: Human
Sample Types: Whole Cells
Applications: BioassayWan Y et al. (2002-12-05), Identification of full, partial and inverse CC chemokine receptor 3 agonists using [35S]GTPgammaS binding. Eur. J. Pharmacol., 2002-1456(1):1-10.
PMID: 12450563
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