P815 Whole Cell Lysate

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-10269

Novus Biologicals, part of Bio-Techne
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NBP2-10269-0.1mg
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Key Product Details

Species

Mouse

Applications

Western Blot

Product Summary for P815 Whole Cell Lysate

GROWTH MEDIA: DMEM & 10% FBS (fetal bovine serum)

Product Specifications

Specificity

P815 (mast cell; Mastocytoma)

Type

Cell

Tissue

Blood Lymph

Tissue Condition

Tumor

Subcellular Fraction

Whole

Formulation, Preparation, and Storage

Formulation

SDS sample buffer containing 50 mM DTT.

Concentration

2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Storage

Store at 4C short term. Aliquot and store at -70C long term. Avoid freeze-thaw cycles.

Background: P815

P815 lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 ug/ml of aprotinin, 5 ug/ml of leupeptin. Cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The P815 lysate was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 50 mM DTT.

Additional P815 Products

Product Documents for P815 Whole Cell Lysate

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for P815 Whole Cell Lysate

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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