Human TIMP Luminex Performance Assay 4-plex Fixed Panel

Name: Human TIMP Luminex Performance Assay 4-plex Fixed Panel
Brand: R&D Systems
SKU: LKTM003

Catalog # LKTM003

Citations (7)

Product Datasheet / COA / SDS

For the measurement of TIMP-1, TIMP-2, TIMP-3 and TIMP-4. Earn double rewards for reviews

Luminex High Performance Assays

Luminex High Performance Assays go through rigorous development, validation, and quality control testing to ensure you are getting the best performance out of your multiplex assay. Panels are customizable so you can order a selection of the analytes or the entire panel.

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Key Product Details

Panel:	Luminex Performance Human Fixed TIMP Magnetic Panel
Assay Type:	Magnetic bead-based multiplex assay for the Luminex^® MAGPIX^® platform
Format:	96-well microplate and antibody-coated magnetic beads
Species:	Human
Analytes Detected:	TIMP-1 TIMP-2 TIMP-3 TIMP-4

Not finding your analyte of interest? View our complete Luminex offering in the full version of our Luminex Assay Customization Tool. We also offer Custom Luminex Services .

View Full Technical Details for Human TIMP Luminex Performance Assay 4-plex Fixed Panel

Human TIMP Luminex Performance Assay 4-plex Fixed Panel Product Features

Magnetic Luminex High Performance Assays are flexible bead-based multiplex assays. They allow up to 50 user-defined target analytes to be simultaneously profiled using cell culture supernates, serum, or plasma samples. Some panels are also validated with human milk, saliva, and/or urine samples.

Simultaneously profile 4 analytes in one sample
Magnetic format allows for easier wash steps
Undergoes similar validation testing as Quantikine® ELISA Kits
Precise and Specific
Requires a small sample volume (<50 µL)
Run your assay in just 3-5 hours
Mass-calibrated standards for consistent results with every new lot of material
Enough reagents for one 96-well plate

Performance Validation

Our Luminex High Performance Assays undergo our most extensive validation testing. All Luminex High Performance Assay Panels are validated for use with serum and plasma samples. Some High Performance Assays are additionally validated for cell culture supernatants, milk, saliva, or urine, as indicated on the individual product data sheets. Luminex High Performance Assays are tested for sensitivity (three-quarters the low standard), intra-assay precision, inter-assay precision, and to ensure assay linearity for validated sample types. Recovery values for individual samples in validated sample types are also tested. Validation data for each analyte can be found in the product datasheet.

Kit Components

Premixed cocktail of antibody-coated Magnetic beads
Premixed cocktail of biotinylated detection antibody
Standard Cocktail(s)
Bead Diluent
Biotin Antibody Diluent
Calibrator Diluent
Wash Buffer
Streptavidin-PE
One flat-bottom 96-well Microplate
Foil Plate Sealers (4)
Mixing Bottle
Standard Value Card**

**A standard curve must be generated each time an assay is run, utilizing values from the Certificate of Analysis provided with each Luminex Assay.

Which Luminex Assay is Right For Me?

Assays for the Luminex platform are offered as High Performance Assays or Discovery Assays . The Luminex High Performance Assays are fully validated panels with a focused selection of analytes. They are available as Configurable User Mix and Premixed options or as Fixed Panels. The Luminex Discovery Assays allow for the maximum number of analytes in a multiplex and is supplied as a premixed kit. View a table comparing the features and benefits of these bead-based multiplex assays on rndsystems.com .

More Information About Magnetic Bead-based Multiplex Kits For The Luminex® Platform

Magnetic Luminex High Performance Assays are designed for use with the Luminex MAGPIX CCD Imager. Alternatively, kits can be used with the Luminex 200™ or FLEXMAP 3D, dual laser, flow-based sorting and detection platforms.

Analyte-specific antibodies are pre-coated onto color-coded microparticles. Microparticles, standards, and samples are pipetted into wells and the immobilized antibodies bind the analytes of interest. After washing away any unbound substances, a biotinylated antibody cocktail specific to the analytes of interest is added to each well. Following a wash to remove any unbound biotinylated antibody, Streptavidin-Phycoerythrin conjugate (Streptavidin-PE), which binds to the biotinylated detection antibodies, is added to each well. A final wash removes unbound Streptavidin-PE and the microparticles are resuspended in buffer and read using a Luminex analyzer. One laser is bead-specific and determines which analyte is being detected. A magnet in the analyzer captures and holds the superparaMagnetic microparticles in a monolayer. Two spectrally distinct Light Emitting Diodes (LEDs) illuminate the beads. One LED identifies the analyte that is being detected and the second LED determines the magnitude of the PE-derived signal, which is in direct proportion to the amount of analyte bound. Each well is imaged with a CCD camera. Kits can also be used with Luminex 100/200 or a FLEXMAP 3D dual laser, flow-based systems.

Human TIMP Luminex Performance Assay 4-plex Fixed Panel Scientific Data Examples

	Human TIMP Luminex Panel Standard Curves
	Human TIMP Performance Luminex Panel Serum Sample Values Serum samples from 24 apparently healthy individuals were run on the Human TIMP Luminex High Performance Panel and sample values plotted. Minimum and maximum values shown by exterior lines, the box represents the 1st and 3rd quartiles with the center line representing the median.

Background: TIMP Assay Kits

TIMPs-1 through -4 regulate the activity of zinc metalloproteases known as MMPs, ADAMs and ADAMTSs. Structurally, TIMPs contain two domains. The N-terminal domain binds to the active site of mature metalloproteases via a 1:1 non-covalent interaction, blocking access of substrates to the catalytic site. In addition, The C-terminal domain of TIMP-1 and TIMP-2 binds to the hemopexin- like domain of pro-MMP-9 and pro-MMP-2, respectively. The latter binding is essential for the cell surface activation of MMP-2 by MMP-14.

Item	Value
Long Name	Tissue Inhibitors of Metalloproteinases 1
Alternate Names	TIMP Assay Kits

Citations for Human TIMP Luminex Performance Assay 4-plex Fixed Panel (7)

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Citations are publications that use Bio-Techne products. Selected citations for Human TIMP Luminex Performance Assay 4-plex Fixed Panel include:

Species: Human
Sample Types: Cell Culture Supernates

C Contant et al. (2021), Anethole induces anti-oral cancer activity by triggering apoptosis, autophagy and oxidative stress and by modulation of multiple signaling pathways Scientific Reports, 11(1):13087.
PMID: 34158560
Species: Squirrel
Sample Types: Tissue Homogenates

SM Logan et al. (2021), Markers of tissue remodeling and inflammation in the white and brown adipose tissues of a model hibernator Cellular Signalling, 82(0):109975.
PMID: 33711429
Species: Human
Sample Types: Plasma

VVS Albuquerqu et al. (2019), Plasma levels of C-reactive protein, matrix metalloproteinase-7 and lipopolysaccharide-binding protein distinguish active pulmonary or extrapulmonary tuberculosis from uninfected controls in children Cytokine, 123(0):154773.
PMID: 31299414
Species: Rabbit
Sample Types: Aqueous Humor

KI Jung et al. (2019), Effects of aqueous suppressants and prostaglandin analogues on early wound healing after glaucoma implant surgery Sci Rep, 9(1):5251.
PMID: 30918313
Species: Human
Sample Types: Cell Culture Supernates

S Niada et al. (2019), Adipose-derived stromal cell secretome reduces TNF?-induced hypertrophy and catabolic markers in primary human articular chondrocytes Stem Cell Res, 38(0):101463.
PMID: 31108390
Species: Human
Sample Types: Plasma

Andrade B et al. (2015), Heme Oxygenase-1 Regulation of Matrix Metalloproteinase-1 Expression Underlies Distinct Disease Profiles in Tuberculosis. J Immunol, 195(6):2763-73.
PMID: 26268658
Species: Human
Sample Types: Cell Culture Supernates

Jonsson S et al. (2014), Overexpression of MMP-9 and its inhibitors in blood mononuclear cells after myocardial infarction--is it associated with depressive symptomatology? PLoS ONE, 9(8):e105572.
PMID: 25153995

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