Human CD40 Ligand/TNFSF5 QuicKit ELISA
R&D Systems, part of Bio-Techne | Catalog # QK1012
Control Set 1249
Key Product Details
Assay Length
90 minutes
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 µL), Serum (10 µL), Platelet-poor EDTA Plasma (50 µL), Platelet-poor Heparin Plasma (50 µL)
Sensitivity
2.43 pg/mL
Assay Range
39.1-2500 pg/mL (Cell Culture Supernates, Serum, Platelet-poor EDTA Plasma, Platelet-poor Heparin Plasma)
Product Summary for Human CD40 Ligand/TNFSF5 QuicKit ELISA
The Quantikine QuicKit Human CD40 Ligand/TNFSF5 Immunoassay is a one-step, 90-minute solid phase ELISA designed to measure human CD40 Ligand levels in cell culture supernates, serum, and platelet-poor plasma. It contains recombinant human CD40 Ligand and antibodies raised against the recombinant protein. Results obtained for naturally occurring human CD40 Ligand showed linear curves that were parallel to the standard curves obtained using the recombinant QuicKit standards. These results indicate that this kit can be used to determine relative mass values for natural human CD40 Ligand.
Product Specifications
Measurement
Quantitative ELISA
Detection Method
Colorimetric - 450nm (TMB)
Conjugate
HRP
Reactivity
Human
Specificity
Natural and recombinant human CD40 Ligand.
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.
Precision
Intra-Assay Precision (Precision within an assay) Two samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Two samples of known concentration were tested in ten separate assays to assess inter-assay precision. Assays were performed by at least five technicians.
Cell Culture Supernates, Platelet-poor EDTA Plasma, Platelet-poor Heparin Plasma, Serum
| Intra-Assay Precision | Inter-Assay Precision | |||
|---|---|---|---|---|
| Sample | 1 | 2 | 1 | 2 |
| n | 20 | 20 | 10 | 10 |
| Mean (pg/mL) | 211 | 1277 | 217 | 1370 |
| Standard Deviation | 8.46 | 31.0 | 20.3 | 99.2 |
| CV% | 4.0 | 2.4 | 9.4 | 7.2 |
Recovery for Human CD40 Ligand/TNFSF5 QuicKit ELISA
The recovery of CD40 Ligand is spiked to three different levels in samples throughout the range of the assay in various matrices was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Media (n=2) | 117 | 112-126 |
| Platelet-poor EDTA Plasma (n=2) | 101 | 90-118 |
| Platelet-poor Heparin Plasma (n=2) | 102 | 87-126 |
| Serum (n=2) | 94 | 85-102 |
Linearity
To assess the linearity of the assay, samples containing and/or spiked with high concentrations of CD40 Ligand were diluted with calibrator diluent to produce samples with values within the dynamic range of the assay.
Scientific Data Images for Human CD40 Ligand/TNFSF5 QuicKit ELISA
Quantikine QuicKit Human CD40 Ligand/TNFSF5 ELISA Standard Curve
Preparation and Storage
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: CD40 Ligand/TNFSF5
CD40 Ligand, also known as TNFSF5, CD154, TRAP, and gp39, is a trimeric protein that is expressed in transmembrane and soluble forms. It is expressed by many immune cell types as well as by endothelial and smooth muscle cells of the vasculature. It binds to CD40 on antigen presenting cells, platelets, and endothelial and epithelial cells, leading to enhanced B cell activation and T cell dependent humoral immune responses. CD40 Ligand dysregulation contributes to immune pathology in AIDS, atherosclerosis, atherothrombosis, and restenosis.
Alternate Names
CD154, CD40L, CD40LG, gp39, TNFSF5, TRAP
Gene Symbol
CD40LG
Additional CD40 Ligand/TNFSF5 Products
Product Documents for Human CD40 Ligand/TNFSF5 QuicKit ELISA
Product Specific Notices for Human CD40 Ligand/TNFSF5 QuicKit ELISA
For research use only
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