Human Exosome (Urine) ELISA Kit (Colorimetric)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-14800

Novus Biologicals, part of Bio-Techne
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Key Product Details

Sample Type & Volume Required Per Well

Human Urine (100 uL)

Sensitivity

< 0.35 ug of overall exosome or < 50 pg targeted exosomes protein

View all Exosome (Urine) ELISA Kits »

Product Specifications

Kit Type

ELISA Kit (Colorimetric)

Reactivity

Human

Description

This ELISA kit ensures dose-dependent exosome detection in a variety of samples.

The quantification can be both relative and absolute as the assay calibration enables the calculation of the approximate exosome quantity for each sample.

Up to 100 uL of biological sample can be loaded per well.

Concentrated urine samples are recommended prior to capture.

Expiration date is 12 months. Stored up to 24 months (if unopened). After opening, buffers and solutions are good for 1 month, plate strips are good for 6 months. After reconstitution, standards can be stored at -20C for 1 month, -80C for 6 months. Avoid Freeze-thaw cycles.

Scientific Data Images for Human Exosome (Urine) ELISA Kit (Colorimetric)

ELISA: Human Exosome (Urine) ELISA Kit (Colorimetric) [NBP3-14800]

ELISA: Human Exosome (Urine) ELISA Kit (Colorimetric) [NBP3-14800]

ELISA: Human Exosome (Urine) ELISA Kit (Colorimetric) [NBP3-14800] - Example of standard curves: CD9 Titration of COLO1 (1) and plasma (2) Exosome Standards. This standard curve is for demonstration only. For quantification purposes a standard curve must be obtained with every assay.

Kit Contents for Human Exosome (Urine) ELISA Kit (Colorimetric)

  • Exosome standards (healthy human plasma)
  • Mouse mAb)
  • Pre-coated ELISA plate (pan exosome)
  • Primary detection antibody (anti-CD9
  • Secondary antibodies (anti-mouse-HRP)
  • Stop solution
  • Substrate solution

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: Exosome (Urine)

Exosomes are small endosome derived lipid nanoparticles (50-120 nm) actively secreted by exocytosis by most living cells. Exosome release occurs either constitutively or upon induction, under both normal and pathological conditions, in a dynamic, regulated and functionally relevant manner. Both the amount and molecular composition of released exosomes depend on the state of a parent cell. Exosomes have been isolated from diverse cell lines (hematopoietic cells, tumor lines, primary cultures, and virus infected cells) as well as from biological fluids in particular blood (e.g. serum and plasma from cancer patients) and other body fluids (broncho alveolar lavage fluid, pleural effusions, synovial fluid, urine, amniotic fluid, semen, saliva etc). Exosomes have pleiotropic physiological and pathological functions and have an emerging role in diverse pathological conditions such as cancer, infectious and neurodegenerative diseases.

Alternate Names

EV detection, exosome detection, extracellular vesicle detection

Additional Exosome (Urine) Products

Product Documents for Human Exosome (Urine) ELISA Kit (Colorimetric)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Human Exosome (Urine) ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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