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N21-MAX Vitamin A Free Media Supplement (50X)

Synaptotagmin Expression in Neurons Cultured in Media Supplemented with N21-MAX Vitamin A Free Media Supplement.
(2)
N21-MAX Vitamin A Free Media Supplement
(2)
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Assay Procedure

Refer to the product datasheet for complete product details.

 

Briefly, completed N21-MAX Vitamin A Free-supplemented medium is prepared using the following procedure:

  • Dilute the media supplement in basal media
  • Store completed media
  • Use within 2 weeks in the dark
 

 

Reagents Provided

Reagents provided in the N21-MAX Vitamin A Free Media Supplement (Catalog # AR012):

  • Albumin (bovine)
  • L-Carnitine
  • Catalase
  • Corticosterone
  • Ethanolamine
  • Glutathione
  • Galactose
  • Holo-Transferrin
  • Insulin
  • Linoleic Acid
  • Lipoic Acid
  • Progesterone
  • Putrescine
  • Selenite
  • Superoxide dismutase
  • Triiodo-L-thyronine
  • D,L-alpha-Tocopherol
  • D,L-alpha-Tocopherol acetate
 

 

Other Supplies Required

Reagents

  • Basal media (e.g., Neurobasal Media from Invitrogen®, Catalog # 21103-029 or equivalent)
  • L-Glutamine

Materials

  • Serological pipettes
  • Pipettes and pipette tips

Equipment

  • 2 °C to 8 °C refrigerator
 

 

Protocol Overview

Dilute 50-fold with a basal media (e.g., Neurobasal Media from Invitrogen, Catalog # 21103-029) and supplement with 0.5 mM L-glutamine before use. The medium may be stored in the dark at 2 °C to 8 °C for up to 2 weeks.

Invitrogen is a registered trademark of Invitrogen Corp.

Citations

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Customer Reviews (2)

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  • N21-MAX Vitamin A Free Media Supplement (50X)
    Name: Anonymous
    Verified Customer | Posted 12/21/2016
    This worked well for neural stem cell expansion.
  • N21-MAX Vitamin A Free Media Supplement (50X)
    Name: Anonymous
    Verified Customer | Posted 12/06/2016
    Used for human iPSC differentiation.

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Product Datasheets

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