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N21-MAX Media Supplement (50X)

N21-MAX Media Supplement (50X) (Catalog # AR008)
(7)
N21-MAX Reduces Spontaneous Stress Rod Formation.
(7)
Improved Resolution of Rod Induction in Neurons Cultured in N21-MAX.
(7)
N21-MAX Media Supplement Enhances Synaptic Development.
(7)
Synaptotagmin and CAM Kinase II Expression in Neurons Cultured in Media Supplemented with N21-MAX.
(7)
Increased Synaptic Puncta and Neurite Outgrowth of Primary Neurons Cultured in N21-MAX.
(7)
N21-MAX Media Supplement Increases Efficiency of Pancreatic Cell Differentiation. 
(7)
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Assay Procedure

Refer to the product datasheet for complete product details.

Briefly, completed N21-MAX-supplemented medium is prepared using the following procedure:

  • Dilute the media supplement in basal media
  • Store completed media
  • Use within 2 weeks

Reagents Provided

Reagents provided in the N21-MAX Media Supplement (Catalog # AR008):

  • Albumin (bovine)
  • L-Carnitine
  • Catalase
  • Corticosterone
  • Ethanolamine
  • Glutathione
  • Galactose
  • Holo-Transferrin
  • Insulin
  • Linoleic Acid
  • Linolenic Acid
  • Lipoic Acid
  • Progesterone
  • Putrescine
  • Retinyl acetate
  • Retinol
  • Selenite
  • Superoxide dismutase
  • Triiodo-L-thyronine
  • D,L-alpha-Tocopherol
  • D,L-alpha-Tocopherol acetate
 

Other Supplies Required

Reagents

  • Basal media (e.g., Neurobasal Media from Invitrogen®, Catalog # 21103-029 or equivalent)
  • L-Glutamine

Materials

  • Serological pipettes
  • Pipettes and pipette tips

Equipment

  • 2 °C to 8 °C refrigerator

 

Protocol Overview

Dilute 50-fold with a basal media (e.g., Neurobasal Media from Invitrogen, Catalog # 21103-029) and supplement with 0.5 mM L-glutamine before use. The medium may be stored in the dark at 2 °C to 8 °C for up to 2 weeks.

 

Invitrogen is a registered trademark of Invitrogen Corp.

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FAQs

  • What advantages does the N21-MAX Media Supplement (Catalog # AR008) have over other neuronal stem cell supplements?

    We have performed comparison testing in-house and have found that in long term cultures (21 days), we observe better synapse marker expression.

    Additionally, one major formulation difference is that our N21 contains holo-transferrin while other supplements typically contain apo-transferrin. It is reported in the literature that holo-transferrin is preferred for neuronal cell culture. We have also tested several BSA sources to determine which is optimal for neuronal cultures.

Product Datasheets

Certificate of Analysis

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