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N21-MAX Insulin Free Media Supplement (50X)

N21-MAX Insulin Free Media Supplement (50X)
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Assay Procedure

Refer to the product datasheet for complete product details.

Briefly, completed N21-MAX Insulin Free-supplemented medium is prepared using the following procedure:

  • Dilute the media supplement in basal media
  • Store completed media
  • Use within 2 weeks in the dark
 

 

Reagents Provided

Reagents provided in the N21-MAX Insulin Free Media Supplement (Catalog # AR010):

  • Albumin (bovine)
  • L-Carnitine
  • Catalase
  • Corticosterone
  • Ethanolamine
  • Glutathione
  • Galactose
  • Holo-Transferrin
  • Linoleic Acid
  • Linolenic Acid
  • Lipoic Acid
  • Progesterone
  • Putrescine
  • Retinyl acetate
  • Retinol
  • Selenite
  • Superoxide dismutase
  • Triiodo-L-thyronine
  • D,L-alpha-Tocopherol
  • D,L-alpha-Tocopherol acetate

 

Other Supplies Required

Reagents

  • Basal media (e.g., Neurobasal Media from Invitrogen®, Catalog # 21103-029 or equivalent)

Materials

  • Serological pipettes
  • Pipettes and pipette tips

Equipment

  • 2 °C to 8 °C refrigerator

 

Procedure Overview

Dilute 50-fold with a basal media (e.g., Neurobasal Media) and supplement with 0.5 mM L-glutamine before use. The medium may be stored in the dark at 2 °C to 8 °C for up to 2 weeks.

 

Invitrogen is a registered trademark of Invitrogen Corp.

Citations

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Product Datasheets

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