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N-2 MAX Media Supplement (100X)

N-2 MAX Media Supplement (100X) (Catalog # AR009)
(5)
Neural Progenitor Cells Expanded with N-2 Plus Media Supplement Express Nestin and SOX2.
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Lot Consistency of N-2 MAX Media Supplement.
(5)
Verification of Neural Progenitor Cell Multipotency Following Expansion with N-2 MAX Media Supplement. 
(5)
N-2 MAX Media Supplement is Comparable to GMP N-2 MAX.
(5)
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Assay Procedure

Refer to the product datasheet for complete product details.

 

Briefly, completed N-2 MAX-supplemented neural cell medium is prepared using the following procedure:

  • Dilute the media supplement in basal media
  • Store completed media
  • Use within 2 weeks
 

 

Reagents Provided

Reagents provided in the N-2 MAX Media Supplement (Catalog # AR009):

  • Recombinant Human Insulin (2,500 µg/mL)
  • Human Transferrin (10,000 µg/mL)
  • Putrescine (1,611 µg/mL)
  • Selenite (0.52 µg/mL)
  • Progesterone (0.63 µg/mL)

 

Other Supplies Required

Reagents

  • DMEM/F-12 (Invitrogen®, Catalog # 12500-062) or a basal media (e.g., Neurobasal Media from Invitrogen, Catalog # 21103-029)
  • Glucose
  • Glutamine
  • NaHCO3
  • Penicillin-Streptomycin (100X)
  • Deionized or distilled water

Materials

  • Serological pipettes
  • Pipettes and pipette tips
  • 2 µm filter unit

Equipment

  • 2 °C to 8 °C refrigerator
 

 

Protocol Overview

Option 1: Mix the following components with deionized or distilled water to make 500 mL of medium.

Component Amount (mg/mL)
DMEM/F-12 6 g
Glucose 0.775 g
Glutamine 0.0365 g
NaHCO3 0.854 g
N-2 MAX Media Supplement 5 mL

 

Adjust the pH to 7.2. Filter the solution (2 µm filter unit), and add 5 mL of 100X sterile Penicillin-Streptomycin solution. The medium may be stored in the dark at 2 °C to 8 °C for up to 2 weeks.

Option 2: Dilute 100-fold with a basal media (e.g., Neurobasal Media from Invitrogen, Catalog # 21103-029) before use. The medium may be stored in the dark at 2 °C to 8 °C for up to 2 weeks.

Invitrogen is a registered trademark of Invitrogen Corp.

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Customer Reviews

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FAQs

  • What is the difference between N-2 Plus Media Supplement (Catalog # AR003) and N-2 MAX Media Supplement (Catalog # AR009)?

    The N-2 Plus Media Supplement (Catalog # AR003) contains Bovine Insulin whereas N-2 MAX Media Supplement (Catalog # AR009) contains Recombinant Human Insulin. All other media components and concentrations in N-2 Plus and N-2 MAX are the same. These two products perform comparably in side-by-side testing. Due to a limited supply of bovine insulin, the products are priced differently.

  • Does N-2 MAX Media Supplement (Catalog # AR009) contain any components that are calcium salts?

    No.
  • Does N-2 MAX Media Supplement (Catalog # AR009) contain any proteins that are produced using Baculovirus?

    No.
  • an the N-2 MAX Media Supplement be stored frozen as 1 mL aliquots?

    Yes. The N-2 MAX Media Supplement may be stored frozen as 1 mL aliquots.  The frozen aliquots should not undergo multiple freeze-thaws before use.

  • Does the N-2 MAX Media Supplement contain any animal-derived components other than Human Transferrin?

    No. The N-2 MAX Media Supplement does not contain any animal-derived components other than Human Transferrin. GMP N-2 MAX Media Supplement is an animal-free supplement (Catalog # AR016).

  • How many freeze/thaw cycles can N-2 MAX Media Supplement (100X), Catalog # AR009, undergo? 

    The N-2 MAX Media Supplement should be able to undergo one freeze/thaw cycyle as long as the product is used witin the expiration date. 

Product Datasheets

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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