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N-2 MAX Media Supplement (100X)

N-2 MAX Media Supplement (100X)_AR009
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Lot Consistency of N-2 MAX Media Supplement
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N-2 MAX Comparable to GMP N-2 MAX_AR009_AR016
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Assay Procedure

Refer to the product datasheet for complete product details.

 

Briefly, completed N-2 MAX-supplemented neural cell medium is prepared using the following procedure:

  • Dilute the media supplement in basal media
  • Store completed media
  • Use within 2 weeks
 

 

Reagents Provided

Reagents provided in the N-2 MAX Media Supplement (Catalog # AR009):

  • Recombinant Human Insulin (2,500 µg/mL)
  • Human Transferrin (10,000 µg/mL)
  • Putrescine (1,611 µg/mL)
  • Selenite (0.52 µg/mL)
  • Progesterone (0.63 µg/mL)

 

Other Supplies Required

Reagents

  • DMEM/F-12 (Invitrogen®, Catalog # 12500-062) or a basal media (e.g., Neurobasal Media from Invitrogen, Catalog # 21103-029)
  • Glucose
  • Glutamine
  • NaHCO3
  • Penicillin-Streptomycin (100X)
  • Deionized or distilled water

Materials

  • Serological pipettes
  • Pipettes and pipette tips
  • 2 µm filter unit

Equipment

  • 2 °C to 8 °C refrigerator
 

 

Protocol Overview

Option 1: Mix the following components with deionized or distilled water to make 500 mL of medium.

Component Amount (mg/mL)
DMEM/F-12 6 g
Glucose 0.775 g
Glutamine 0.0365 g
NaHCO3 0.854 g
N-2 MAX Media Supplement 5 mL

 

Adjust the pH to 7.2. Filter the solution (2 µm filter unit), and add 5 mL of 100X sterile Penicillin-Streptomycin solution. The medium may be stored in the dark at 2 °C to 8 °C for up to 2 weeks.

Option 2: Dilute 100-fold with a basal media (e.g., Neurobasal Media from Invitrogen, Catalog # 21103-029) before use. The medium may be stored in the dark at 2 °C to 8 °C for up to 2 weeks.

Invitrogen is a registered trademark of Invitrogen Corp.

Citations (13)

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Customer Reviews (10)

4.7 out of 5
10 customer ratings
5 stars
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30%
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  • N-2 MAX Media Supplement (100X)
    Name: Anonymous
    Verified Customer | Posted 12/28/2020
    Cultured cells with N2 supplement and they grew well..
    N-2 MAX Media Supplement (100X) Serum AR009
  • N-2 MAX Media Supplement (100X)
    Name: Anonymous
    Verified Customer | Posted 01/30/2020
    Differentiated human iPS cells into NPCs
    N-2 MAX Media Supplement (100X) Serum AR009
  • N-2 MAX Media Supplement (100X)
    Name: Anonymous
    Verified Customer | Posted 08/15/2019
    We cultured with N2 supplement.It's good for cell growth.
    N-2 MAX Media Supplement (100X) Serum AR009
  • N-2 MAX Media Supplement (100X)
    Name: Anonymous
    Verified Customer | Posted 07/07/2018
    N-2 MAX Media Supplement (100X) Serum AR009
  • N-2 MAX Media Supplement (100X)
    Name: Anonymous
    Verified Customer | Posted 11/01/2017
    IPSCs cultured on a polymer supported plate for differentiation
    N-2 MAX Media Supplement (100X) Serum AR009
  • N-2 MAX Media Supplement (100X)
    Name: Anonymous
    Verified Customer | Posted 05/30/2017
    I have previously used N2 Neuroplex supplement from Gemimi Bio (Cat # 400-163) to culture neurons and this sample from R&D Systems work equally well. We will be switching to N-2 MAX.
    N-2 MAX Media Supplement (100X) Serum AR009
  • N-2 MAX Media Supplement (100X)
    Name: Anonymous
    Verified Customer | Posted 12/06/2016
    N2 Max is a component of our base media for differentiating mouse and human stem cells to neural progenitor cells for modeling neural development and disease.
  • N-2 MAX Media Supplement (100X)
    Name: Anonymous
    Verified Customer | Posted 12/06/2016
    I use this product to grow Neural Progenitor Cells (NPC). This product is excellent. The cells grow very well in this media.
  • N-2 MAX Media Supplement (100X)
    Name: Anonymous
    Verified Customer | Posted 12/06/2016
    We use in our media for neural stem cell expansion.
  • N-2 MAX Media Supplement (100X)
    Name: Anonymous
    Verified Customer | Posted 12/06/2016
    N2 max supplement was used throughout the whole process of generation, culture, and maintenance of human and mouse iPS-derived dopaminergic neurons. The product performed well and better than one produced by another company.

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Showing 1- 5 of 10 reviews Showing All

FAQs

  • What is the difference between N-2 Plus Media Supplement (Catalog # AR003) and N-2 MAX Media Supplement (Catalog # AR009)?

    The N-2 Plus Media Supplement (Catalog # AR003) contains Bovine Insulin whereas N-2 MAX Media Supplement (Catalog # AR009) contains Recombinant Human Insulin. All other media components and concentrations in N-2 Plus and N-2 MAX are the same. These two products perform comparably in side-by-side testing. Due to a limited supply of bovine insulin, the products are priced differently.

  • Does N-2 MAX Media Supplement (Catalog # AR009) contain any components that are calcium salts?

    No.

  • Does N-2 MAX Media Supplement (Catalog # AR009) contain any proteins that are produced using Baculovirus?

    No.

  • an the N-2 MAX Media Supplement be stored frozen as 1 mL aliquots?

    Yes. The N-2 MAX Media Supplement may be stored frozen as 1 mL aliquots.  The frozen aliquots should not undergo multiple freeze-thaws before use.

  • Does the N-2 MAX Media Supplement contain any animal-derived components other than Human Transferrin?

    No. The N-2 MAX Media Supplement does not contain any animal-derived components other than Human Transferrin. GMP N-2 MAX Media Supplement is an animal-free supplement (Catalog # AR016).

  • How many freeze/thaw cycles can N-2 MAX Media Supplement (100X), Catalog # AR009, undergo? 

    The N-2 MAX Media Supplement should be able to undergo one freeze/thaw cycyle as long as the product is used witin the expiration date. 

Product Datasheets

Certificate of Analysis

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