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ITS Media Supplement (100X)

Insulin-Transferrin-Selenium Media Supplement
ITS Media Supplement Supports Proliferation Under Low Serum Conditions.
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Assay Procedure

Refer to the product datasheet for complete product details.

Briefly, ITS Media Supplement is prepared using the following procedure:

  • Dilute the media into the cell culture media of interest
  • Store completed media at 2 – 8 °C


Reagents Provided

Reagents provided in the ITS Media Supplement (100X) (Catalog # AR013):

Component Amount (µg/mL)
Recombinant Human Insulin 1000
Human Transferrin 500
Selenite 0.67


Stability and Storage

Store in the dark at < -20 °C in a manual defrost freezer. Do not use past the expiration date.


This product contains human transferrin. This transferrin was tested at the donor level using an FDA licensed method and found to be non-reactive for anti-HIV-1/2 and Hepatitis B surface antigen. As no testing can offer complete assurance of freedom from infectious reagents, these reagents should be handled as if capable of transmitting infection.


Other Supplies Required


  • Basal media
  • Serological pipettes
  • Pipettes and pipette tips


  • 2 °C to 8 °C refrigerator


Procedure Overview

Dilute 100-fold into cell culture media of interest before use. The final concentration of serum required with ITS supplementation is cell type and assay dependent. Titrate for individual use.

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Product Datasheets

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