Proteome Profiler Human Protease Array Kit Summary
R&D Systems Proteome Profiler™ Human Protease Array Kit (Catalog # ARY021) is a rapid, sensitive, and economical tool to simultaneously detect the relative changes of 34 proteases in a single sample. No specialized equipment is necessary.
Simultaneously detect the relative levels of these proteases in a single sample.
Proprotein Convertase 9
General Assay Principle
Carefully selected capture antibodies have been spotted in duplicate on nitrocellulose membranes. Cell culture supernates, cell lysates, serum, plasma, urine, saliva, or tissue lysate samples are diluted and mixed with a cocktail of biotinylated detection antibodies. The sample/antibody mixture is then incubated with the array. Any protein/detection antibody complex present is bound by its cognate immobilized capture antibody on the membrane. Streptavidin-Horseradish Peroxidase and chemiluminescent detection reagents are added, and a signal is produced in proportion to the amount of protein bound. Chemiluminescence is detected in the same manner as a Western blot.
4 Array Membranes
Detection Antibody Cocktail
Chemiluminescent Detection Reagents
Transparency Overlay Template
For a complete list of the kit contents and necessary materials, please see the Materials Provided/Other Supplies Required sections of the product datasheet.
Preparation & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Which Brands are Currently Available on bio‑techne.com? R&D Systems, Tocris Bioscience and ProteinSimple branded products are available to purchase through bio‑techne.com. ProteinSimple branded instruments are available to quote. ACD branded products will be available on bio‑techne.com in the near future. Novus Biologicals branded products are not currently available on bio‑techne.com and can be found at novusbio.com.
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