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TRACP/PAP/ACP5 Antibody (220), Novus Biologicals

Bio-Techne includes Novus Biologicals | Catalog # NBP3-11295

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NBP3-11295

Key Product Details

Species Reactivity

Human

Applications

Immunoassay, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 220

Concentration

0.1 mg/ml

Product Summary for TRACP/PAP/ACP5 Antibody (220)

Immunogen

Recombinant TRACP

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Applications for TRACP/PAP/ACP5 Antibody (220)

Application
Recommended Usage

Immunoassay

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Paraffin

Optimal dilutions of this antibody should be experimentally determined.

Immunoprecipitation

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
For immunohistochemistry, immunoprecipitation, Immunoassays and Western blotting, dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50. For functional studies, in vitro dilutions have to be optimized in user's experimental setting.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

0.2 um filtered solution in PBS, 0.1% BSA

Preservative

0.02% Sodium Azide

Concentration

0.1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: TRACP/PAP/ACP5

Monoclonal antibody 220 recognizes human tartrate-resistant-acid phosphatase 5a (TRACP5a). TRACP is considered a marker for differentiated monocytes like macrophages, dendritic cells and osteoclasts. It is also considered to be a serum marker for bone disease. TRACP has two isotypes (5a &5b) with different characteristics. Isoform 5a is intact and bears a sialic acid and has a lower activity and pH optimum (pH5.0-5.2) than TRACP5b (pH5.8-6.0). TRACP5a circulates as a 35kD glycoprotein and 5b as a linked heterodimer of proteolytically cleaved16 & 23 kDa fragments. TRACP5a has unique epitopes associated with the intact loop peptide. Antibody 220 recognizes the regulatory loop peptide which interacts with the active site of TRACP5a. Serum TRACP protein is predominantly isoform 5a, while most of the activity is due to isoform 5b. TRACP5a, but not 5b is secreted by activated macrophages and dendritic cells and is therefore considered a marker for systemic macrophage and chronic inflammation. TRACP 5a levels are correlated positively with inflammation-related markers CRP, ferritin, and triglycerides, which are also elevated in cardiovascular patients (CVD), but have no relationship to TRAP 5b activity or other bone metabolic markers. TRACP5a is a specific secreted marker of inflammatory macrophages relevant to their systemic number. TRACP5b is a marker for osteoclast number and bone resorption. The clinical significance of TRACP5a is not completely clear yet. TRACP5a levels are elevated in end stage renal disease, 30% of rheumatoid arthritis patients and was positively correlated with body mass and fat indices (adipose tissue macrophages). Elevated serum TRACP5a may be an indicator of advanced or systemic inflammatory disease associated with inflammatory vulnerable plaque and a risk marker for adverse events in CVD.

Long Name

Tartrate Resistant Acid Phosphatase 5

Alternate Names

ACP5, TRACP-5b, TTRRAP

Gene Symbol

ACP5

Additional TRACP/PAP/ACP5 Products

Product Documents for TRACP/PAP/ACP5 Antibody (220)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for TRACP/PAP/ACP5 Antibody (220)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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