TGF-beta 1, 2, 3 PE-conjugated Antibody

Name: TGF-beta 1, 2, 3 PE-conjugated Antibody
Brand: R&D Systems
SKU: IC1835P

R&D Systems, Inc. a Bio-Techne Brand

Catalog # IC1835P

Citations (3)

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Key Product Details

Immunogen:	Bovine bone-derived TGF-beta 1 and TGF-beta 2
Applications:	Flow Cytometry
Label:	Phycoerythrin (Excitation=488 nm, Emission=565-605 nm)
Source:	Monoclonal Mouse IgG₁ Clone # 1D11
Specificity:	Detects bovine, chicken, mouse, and human TGF-beta in ELISAs and Western blots. It recognizes human TGF-beta 1, TGF-beta 2, and TGF-beta 3.

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Applications for TGF-beta 1, 2, 3 PE-conjugated Antibody

	Recommended Concentration	Sample
Intracellular Staining by Flow Cytometry	10 µL/10⁶ cells	See below

Please Note: Optimal dilutions should be determined by each laboratory for each application.

Scientific Data Examples for TGF-beta 1, 2, 3 PE-conjugated Antibody

	Intracellular Staining by Flow Cytometry Detection of TGF‑ beta 1, 2, 3 in PC‑3 Human Cell Line by Flow Cytometry. PC-3 human prostate cancer cell line was stained with Mouse Anti-TGF-beta 1, 2, 3 PE-conjugated Mono-clonal Antibody (Catalog # IC1835P, filled histogram) or isotype control antibody (Catalog # IC002P, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

Formulation, Preparation and Storage

Purification	Protein A or G purified from hybridoma culture supernatant
Formulation	Supplied in a saline solution containing BSA and Sodium Azide.
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied.

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Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

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Background: TGF-beta 1, 2, 3

TGF-beta 1, -2, and -3 are a closely related group of proteins (70‑80% sequence homology) that are produced by many cell types and function as growth and differentiation factors. The active forms of TGF-beta 1, -2, and -3 are disulfide-linked homodimers.

Item	Value
References	Ayala A. et al. (1992) FASEB J. 6:A1604. Roberts A.B. and Sporn M.B., eds. (1990) Peptide Growth Factors and Their Receptors I, Springer-Verlag, 419. Dasch J.R. et al. (1989) J. Immunol. 142:1536.
Long Name	Transforming Growth Factor beta 1, 2, and 3
Alternate Names	CED, DPD1, LAP, TGF-beta 1, 2, 3, TGFB, TGFB1, TGFbeta, transforming growth factor, beta 1

Citations for TGF-beta 1, 2, 3 PE-conjugated Antibody (3)

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Citations are publications that use Bio-Techne products. Selected citations for TGF-beta 1, 2, 3 PE-conjugated Antibody include:

Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry

M Beki? et al. (2022), Phosphonate-Modified Cellulose Nanocrystals Potentiate the Th1 Polarising Capacity of Monocyte-Derived Dendritic Cells via GABA-B Receptor International Journal of Nanomedicine, 17(0):3191-3216.
PMID: 35909813
Species: Mouse
Sample Types: Exosomes
Applications: Flow Cytometry

Yu L et al. (2013), Exosomes with membrane-associated TGF-beta1 from gene-modified dendritic cells inhibit murine EAE independently of MHC restriction. Eur J Immunol, 43(9):2461-72.
PMID: 23716181
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry

Notley CA et al. (2011), Natural IgM is required for suppression of inflammatory arthritis by apoptotic cells. J. Immunol., 186(8):4967-72.
PMID: 21383247

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Product Documents for TGF-beta 1, 2, 3 PE-conjugated Antibody

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TGF-beta 1, 2, 3 PE-conjugated Antibody

R&D Systems, Inc. a Bio-Techne Brand

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Applications for TGF-beta 1, 2, 3 PE-conjugated Antibody

Scientific Data Examples for TGF-beta 1, 2, 3 PE-conjugated Antibody

Intracellular Staining by Flow Cytometry