PRAME Antibody (CL5148) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-44111

Novus Biologicals, part of Bio-Techne

Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Human

Applications

Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # CL5148

Format

Azide and BSA Free

Concentration

LYOPH mg/ml

View all PRAME Antibodies »

Product Specifications

Immunogen

This antibody was generated using a recombinant protein sequence of P78395, with the exact immunogen sequence remaining proprietary.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Description

Novus Biologicals Mouse PRAME Antibody (CL5148) - Azide and BSA Free (NBP2-61149) is a monoclonal antibody validated for use in IHC and WB. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for PRAME Antibody (CL5148) - Azide and BSA Free

PRAME Antibody (CL5148) - Azide and BSA Free Immunohistochemistry-Paraffin: PRAME Antibody [NBP3-44111]

Immunohistochemistry-Paraffin: PRAME Antibody [NBP3-44111]

Analysis in human testis and cervix, uterine tissues using NBP3-44111 antibody. Corresponding PRAME RNA-seq data are presented for the same tissues.
PRAME Antibody (CL5148) - Azide and BSA Free Immunohistochemistry-Paraffin: PRAME Antibody [NBP3-44111]

Immunohistochemistry-Paraffin: PRAME Antibody [NBP3-44111]

Staining of human cerebral cortex shows no positivity in neurons as expected.
PRAME Antibody (CL5148) - Azide and BSA Free Immunohistochemistry-Paraffin: PRAME Antibody [NBP3-44111]

Immunohistochemistry-Paraffin: PRAME Antibody [NBP3-44111]

Staining of human endometrium shows moderate nuclear positivity in glandular cells.
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Applications for PRAME Antibody (CL5148) - Azide and BSA Free

Application
Recommended Usage

Immunohistochemistry-Paraffin

1:50 - 1:200

Western Blot

1 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Formulation, Preparation, and Storage

Purification

Protein A purified

Reconstitution

Centrifuge the vial of lyophilized antibody at 12,000 x g for 20 seconds. Reconstitute by adding sterile, distilled water to achieve a final antibody concentration of 1mg/ml.

Formulation

Lyophilized from a 0.2 um filtered solution in PBS with Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: PRAME

PRAME encodes an antigen that is predominantly expressed in human melanomas and that is recognized by cytolytic T lymphocytes. It is not expressed in normal tissues, except testis. This expression pattern is similar to that of other CT antigens, such as MAGE, BAGE and GAGE. However, unlike these other CT antigens, this gene is also expressed in acute leukemias. Recent data from Epping et.al., (Cell, Vol. 122: 835) suggest that overexpression of PRAME frequently observed in human cancers confers growth or survival advantages by antagonizing RAR signaling.

Alternate Names

cancer/testis antigen 130, MAPEOIP4CT130, melanoma antigen preferentially expressed in tumors, OIP-4, Opa-interacting protein 4, Opa-interacting protein OIP4, preferentially expressed antigen in melanoma, Preferentially expressed antigen of melanoma

Gene Symbol

PRAME

Additional PRAME Products

Product Documents for PRAME Antibody (CL5148) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for PRAME Antibody (CL5148) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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