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Mouse/Rat CD31/PECAM-1 Antibody

Detection of Mouse CD31/PECAM-1 antibody by Western Blot.
(6)
Detection of CD31/PECAM-1 antibody in Mouse Splenocytes antibody by Flow Cytometry.
Detection of CD31/PECAM-1 antibody in Rat Splenocytes antibody by Flow Cytometry.
CD31/PECAM-1 antibody in bEnd by Immunocytochemistry (ICC).3 Mouse Cell Line by Immunocytochemistry (ICC).
CD31/PECAM-1 antibody in Mouse Embryo by Immunohistochemistry (IHC-Fr).
CD31/PECAM-1 antibody in Mouse Embryo by Immunohistochemistry (IHC-Fr).
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AF3628-SP
AF3628

Key Product Details

Species Reactivity: Mouse, Rat
Immunogen: Mouse myeloma cell line NS0-derived recombinant mouse CD31/PECAM-1
Glu18-Lys590
Accession # Q08481
Applications: CyTOF-ready, Flow Cytometry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry, Western Blot
Label: Unconjugated
Source: Polyclonal Goat IgG
Specificity: Detects mouse CD31/PECAM-1 in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 10% cross-reactivity with recombinant human CD31 and recombinant porcine CD31 is observed. Detects mouse CD31 and rat CD31 in flow cytometry.

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Customer Reviews (11)

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  • Mouse/Rat CD31/PECAM-1 Antibody
    Name: Anonymous
    Application: ICC/IF
    Sample Tested: Brown adipose tissue
    Species: Mouse
    Verified Customer | Posted 12/09/2020
    Detection of CD31/PECAM-1 in Mouse brown adipose tissue. 4% PFA fixed Brown adipose tissue was embedded in OCT and cryosectioned at 10µm. After permeabilization and blocking, tissue was incubated overnight at 4°C with 2µg/ml of gt@CD31 (AF3628) and detected with Alexa fluor dk@gt 488 (1:1000) the next day. CD31 is presented in pseudo color violet here. This product is great, always very clear and bright. I often use it with 488 channel in tissues with strong autofluorescence such as hearts and quadriceps.
    Mouse/Rat CD31/PECAM-1 Antibody Immunocytochemistry/Immunofluorescence AF3628
  • Mouse/Rat CD31/PECAM-1 Antibody
    Name: Anonymous
    Application: ICC/IF
    Sample Tested: E12.5 mouse embryo fixed in 4% PFA
    Species: Mouse
    Verified Customer | Posted 12/08/2020
    One of the best antibodies.Used on E9.5-E12.5 cryo embedded mouse embryo sections (4% PFA fixed).Attached picture is E12.5 embryo section.Dilution used - 1:200
    Mouse/Rat CD31/PECAM-1 Antibody Immunocytochemistry/Immunofluorescence AF3628
  • Mouse/Rat CD31/PECAM-1 Antibody
    Name: Anonymous
    Application: ICC/IF
    Sample Tested: Adult brain
    Species: Mouse
    Verified Customer | Posted 11/20/2020
    Mouse/Rat CD31/PECAM-1 Antibody Immunocytochemistry/Immunofluorescence AF3628
  • Mouse/Rat CD31/PECAM-1 Antibody
    Name: Anonymous
    Application: IHC
    Sample Tested: Brain (cortex) tissue,Brain (hippocampus)
    Species: Rat
    Verified Customer | Posted 01/21/2020
    Mouse/Rat CD31/PECAM-1 Antibody Immunohistochemistry AF3628
  • Mouse/Rat CD31/PECAM-1 Antibody
    Name: Anonymous
    Application: IHC
    Sample Tested: Liver tissue
    Species: Rat
    Verified Customer | Posted 08/20/2018
    FFPE rat liver stained using AF3628 at 15 µg/mL overnight at 4C. Epitope retrieval was performed at 95 C for 15 minutes in citrate buffer pH 6.0. Secondary was donkey anti-goat Alexa Fluor 555 (psuedocolored red).
    Mouse/Rat CD31/PECAM-1 Antibody Immunohistochemistry AF3628
  • Mouse/Rat CD31/PECAM-1 Antibody
    Name: Anonymous
    Application: CLARITY deep tissue imaging
    Sample Tested: Adult kidney
    Species: Mouse
    Verified Customer | Posted 06/02/2018
    Optically cleared 1 mm mouse kidney sectionImaged by diSPIM in 60% TDE with 17X lensSecondary antibody is donkey anti goat alexa 488Red is Tubulin Beta 3 stained by alexa 568
    Mouse/Rat CD31/PECAM-1 Antibody CLARITY deep tissue imaging AF3628
  • Mouse/Rat CD31/PECAM-1 Antibody
    Name: Anonymous
    Application: ICC/IF
    Sample Tested: Adult brain
    Species: Mouse
    Verified Customer | Posted 04/05/2018
    Excellent!
    Mouse/Rat CD31/PECAM-1 Antibody Immunocytochemistry/Immunofluorescence AF3628
  • Mouse/Rat CD31/PECAM-1 Antibody
    Name: Anonymous
    Application: IHC
    Sample Tested: Liver tissue
    Species: Mouse
    Verified Customer | Posted 03/26/2018
    FFPE mouse liver stained O/N at 4C with AF3826 at 10 ug/mL in 0.3% PBS-Tx. Secondary Donkey anti-goat Alexa Fluor 488 was applied for 1 hr RT at 2 ug/mL in PBS-Tx. Citrate buffer pH 6.0 used for epitope retrieval.
    Mouse/Rat CD31/PECAM-1 Antibody Immunohistochemistry AF3628
  • Mouse/Rat CD31/PECAM-1 Antibody
    Name: Anonymous
    Application: IHC
    Sample Tested: Embryonic kidney
    Species: Mouse
    Verified Customer | Posted 09/12/2017
    Mouse/Rat CD31/PECAM-1 Antibody Immunohistochemistry AF3628
  • Mouse/Rat CD31/PECAM-1 Antibody
    Name: Anonymous
    Application: IHC-P
    Sample Tested: Formalin Fixed Paraffin Embedded Mouse Liver
    Species: Mouse
    Verified Customer | Posted 06/29/2017
    WD [1:500], HIER with DIVA; secondary ab RTU Polymer-HRP
    Mouse/Rat CD31/PECAM-1 Antibody Immunohistochemistry-Paraffin AF3628
  • Mouse/Rat CD31/PECAM-1 Antibody
    Name: Anonymous
    Application: IHC
    Sample Tested: Embryo tissue
    Species: Mouse
    Verified Customer | Posted 08/08/2016
    E9.5 mouse embryonic tissue, whole mount staining with 1:100 dilution, Alex Fluor 594 donkey anti-goat with 1:400 dilution.
    Mouse/Rat CD31/PECAM-1 Antibody Immunohistochemistry AF3628

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