Mouse IL-27 Alexa Fluor® 350-conjugated Antibody

Name: Mouse IL-27 Alexa Fluor® 350-conjugated Antibody
Brand: R&D Systems
SKU: IC18342U

R&D Systems, Inc. a Bio-Techne Brand

Catalog # IC18342U

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Key Product Details

Species Reactivity:	Mouse
Immunogen:	Mouse myeloma cell line NS0-derived recombinant mouse IL-27 heterodimer Tyr19-Pro228 (EBI3), Phe29-Ser234 (p28) Accession # O35228 (EBI3) & Q8K3I6 (p28)
Applications:	Flow Cytometry
Label:	Alexa Fluor 350 (Excitation=346 nm, Emission=442 nm)
Specificity:	Detects mouse IL-27 in direct ELISAs. In direct ELISAs, approximately 10% cross-reactivity with recombinant mouse (rm) IL‑27 p28, rmIL‑27 EBI3, recombinant human (rh) IL-27 p28, and rhIL-27 EBI3 is observed.

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Applications for Mouse IL-27 Alexa Fluor® 350-conjugated Antibody

	Recommended Concentration	Sample
Intracellular Staining by Flow Cytometry	0.25-1 µg/10⁶ cells	Mouse splenocytes treated with LPS, fixed with paraformaldehyde and permeabilized with saponin

Please Note: Optimal dilutions should be determined by each laboratory for each application.

Formulation, Preparation and Storage

Formulation	Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store the unopened product at 2 - 8 °C. Do not use past expiration date.

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Background: IL-27

Interleukin 27 (IL-27) is a secreted heterodimeric cytokine comprised of the IL-12 p35-related protein, p28, and the IL-12 p40-related protein, EBI3 (Epstein-Barr virus‑induced gene 3). IL-27 is expressed by monocytes, endothelial cells and dendritic cells. It binds TCCR/WSX-1 on naïve CD4⁺ T cells and induces the expression of a functional IL-12 receptor, allowing IL-12-induced Th1 polarization. Human EBI3 is 61% amino acid (aa) identical to mouse EBI3 and includes an 20 aa signal peptide and a 209 aa mature protein with two fibronectin type III domains.

Item	Value
Long Name	Interleukin 27
Entrez Gene IDs	246778 (Human); 246779 (Mouse); 102121242 (Cynomolgus Monkey)
Alternate Names	IL-27, IL-27 p28 subunit, IL-27 subunit alpha, IL-27-A, IL27, IL27-A, IL27A, IL27p28, IL30, MGC71873, interleukin 27, interleukin 30, interleukin-27 subunit alpha, p28IL-27A

Product Specific Notices for Mouse IL-27 Alexa Fluor® 350-conjugated Antibody

This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.

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Mouse IL-27 Alexa Fluor® 350-conjugated Antibody

R&D Systems, Inc. a Bio-Techne Brand

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Applications for Mouse IL-27 Alexa Fluor® 350-conjugated Antibody

Formulation, Preparation and Storage

Plan Your Experiments

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Background: IL-27

Product Specific Notices for Mouse IL-27 Alexa Fluor® 350-conjugated Antibody

Citations for Mouse IL-27 Alexa Fluor® 350-conjugated Antibody

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