Human PDGF-BB Biotinylated Antibody

Name: Human PDGF-BB Biotinylated Antibody
Brand: R&D Systems
SKU: BAF220
Rating: 5 (2 reviews)

R&D Systems, Inc. a Bio-Techne Brand

Catalog # BAF220

Citations (2)

(2)

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Key Product Details

Species Reactivity:	Human
Immunogen:	E. coli-derived recombinant human PDGF‑BB (R&D Systems, Catalog # 220-BB) Ser82-Thr190 Accession # Q6FHE7
Applications:	ELISA Detection, Western Blot
Label:	Biotin
Source:	Polyclonal Goat IgG
Specificity:	Detects human PDGF-BB in ELISAs and Western blots. In sandwich immunoassays, approximately 50% cross-reactivity with recombinant rat PDGF‑BB and porcine PDGF is observed, less than 2% cross-reactivity with recombinant human (rh) PDGF‑AB is observed, and less than 0.8% cross-reactivity with rhPDGF‑AA is observed.

View Full Technical Details for Human PDGF-BB Biotinylated Antibody

Applications for Human PDGF-BB Biotinylated Antibody

	Recommended Concentration	Sample
Western Blot	0.1 µg/mL	Recombinant Human PDGF-BB (Catalog # 220-BB)

Human PDGF-BB Sandwich Immunoassay

Recommended Concentration

Reagent

ELISA Detection (Matched Antibody Pair)

0.1-0.4 µg/mL

Use in combination with:

Capture Reagent: Recombinant Human PDGF R beta Fc Chimera Protein, CF (Catalog # 385-PR/CF)

Standard: Recombinant Human PDGF-BB Protein, CF (Catalog # 220-BB)

Please Note: Optimal dilutions should be determined by each laboratory for each application.

Formulation, Preparation and Storage

Purification	Antigen Affinity-purified
Reconstitution	Reconstitute at 0.2 mg/mL in sterile PBS. Reconstitution Buffer Available: Size / Price Qty Loading... RB01:
Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.

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Background: PDGF-BB

Platelet-derived growth factor (PDGF) was discovered as a major mitogenic factor present in serum but absent from plasma. It was found to be secreted from the alpha ‑granules of platelets activated during the coagulation of blood to form serum. Subsequent studies have demonstrated that PDGF is not one molecule but three, each a dimeric combination of two distinct but structurally related peptide chains designated A and B. The dimeric isoforms PDGF-AA, AB and BB are differentially expressed in various cell types and their effects are mediated through two distinct receptors, termed alpha and beta. Differences exist in isoform binding to each receptor. In general, PDGF isoforms are potent mitogens for connective tissue cells, including dermal fibroblasts, glial cells, arterial smooth muscle cells and some epithelial and endothelial cells. In addition to its activity as a mitogen, PDGF is chemotactic for fibroblasts, smooth muscle cells, neutrophils and mononuclear cells. Other reported activities for PDGF include stimulation of granule release by neutrophils and monocytes, facilitation of steroid synthesis by Leydig cells, stimulation of neutrophil phagocytosis, inhibition of natural killer (NK) cell activity, stimulation of collagen synthesis, modulation of thrombospondin expression and secretion, stimulation of collagenase activity and secretion, induction of contraction of rat aorta strips in vitro, and transient induction of T cell IL-2 secretion accompanied by a down-regulation of IL-4 and IFN-gamma production, temporary effects that may allow clonal expansion of antigen-activated B and T helper lymphocytes prior to differentiation. PDGF also appears to be ubiquitous in neurons throughout the CNS, where it is suggested to play an important role in neuron survival and regeneration, and in mediation of glial cell proliferation and differentiation.

Item	Value
Long Name	Platelet-derived Growth Factor BB
Entrez Gene IDs	5155 (Human); 24628 (Rat)
Alternate Names	PDGF-BB, PDGFBB

Citations for Human PDGF-BB Biotinylated Antibody (2)

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Citations are publications that use Bio-Techne products. Selected citations for Human PDGF-BB Biotinylated Antibody include:

Species: Human
Sample Types: Cell Lysates
Applications: TaqMan Protein Assay

(2012), Vascular endothelial growth factor signals through platelet-derived growth factor receptor beta in meningiomas in vitro. Br. J. Cancer, 107(10):1702-13.
PMID: 23047550
Species: Human
Sample Types: Cell Culture Supernates
Applications: ELISA Development

Lacoste E et al. (2003), Platelet concentrates: effects of calcium and thrombin on endothelial cell proliferation and growth factor release. J. Periodontol., 74(10):1498-507.
PMID: 14653397

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Customer Reviews for Human PDGF-BB Biotinylated Antibody (2)

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Human PDGF-BB Biotinylated Antibody

Name: Anonymous

Application: ELISA

Sample Tested: Serum and Plasma

Species: Human

Verified Customer | Posted 07/19/2019
Human PDGF-BB Biotinylated Antibody

Name: Anonymous

Application: ELISA

Sample Tested: Serum and Plasma

Species: Human

Verified Customer | Posted 11/16/2017

This antibody was used as a detection molecule in an sandwich ELISA measuring PDGF in human plasma samples.

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Human PDGF-BB Biotinylated Antibody

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Applications for Human PDGF-BB Biotinylated Antibody

Human PDGF-BB Sandwich Immunoassay

Formulation, Preparation and Storage

Reconstitution Calculator

Background: PDGF-BB

Citations for Human PDGF-BB Biotinylated Antibody (2)

Customer Reviews for Human PDGF-BB Biotinylated Antibody (2)

Customer Images

Human PDGF-BB Biotinylated Antibody

Human PDGF-BB Biotinylated Antibody

Product Documents for Human PDGF-BB Biotinylated Antibody

Product Datasheets

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