Human/Mouse/Rat TrkB Alexa Fluor® 488-conjugated Antibody

Name: Human/Mouse/Rat TrkB Alexa Fluor® 488-conjugated Antibody
Brand: R&D Systems
SKU: FAB397G

R&D Systems, part of Bio-Techne | Catalog # FAB397G

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Product Datasheet / COA

Key Product Details

Species Reactivity:	Human, Mouse, Rat
Immunogen:	Mouse myeloma cell line NS0-derived recombinant human TrkB Cys32-His430 Accession # Q16620
Applications:	Immunohistochemistry, Western Blot
Label:	Alexa Fluor 488 (Excitation=488 nm, Emission=515-545 nm)
Specificity:	Detects human TrkB in direct ELISAs. Detects human, mouse, and rat TrkB in Western blots. In ELISAs and Western blots, no cross-reactivity with recombinant human (rh) TrkA, rhTrkC, or recombinant rat TrkA is observed.

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Technical Data
Citations / Reviews

Applications

	Recommended Concentration	Sample
Western Blot	Optimal dilution of this antibody should be experimentally determined.
Immunohistochemistry	Optimal dilution of this antibody should be experimentally determined.

Please Note: Optimal dilutions should be determined by each laboratory for each application.

Formulation, Preparation and Storage

Purification	Protein A or G purified
Formulation	Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

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Background: TrkB

The neurotrophins, including NGF, BDNF, NT-3, and NT-4/5 constitute a group of structurally related, secreted proteins that play an important role in the development and function of the nervous system. The biological activities of the neurotrophins are mediated by binding to the different members of the Trk family tyrosine kinase receptors. Three Trk family proteins, TrkA, TrkB, and TrkC, exhibiting different ligand specificities, have been identified. TrkA binds NGF, TrkB binds BDNF and NT‑4/5 and TrkC binds NT-3. All Trk family proteins share a conserved complex subdomain organization consisting of a signal peptide, two cysteine-rich domains, a cluster of three leucine-rich motifs, and two immunoglobulin-like domains in the extracellular region, as well as an intracellular region that contains the tyrosine kinase domain. Natural splice variants of the different Trks, including TrkB variants lacking the first cysteine-rich domain, the first and second or all three of the leucine-rich motifs, or the tyrosine kinase domain, have been described. The role of the different extracellular subdomains of TrkB in mediating neurotrophin binding and discrimination is currently being investigated. At the protein sequence level, human and rat TrkB are greater than 90% identical and the proteins exhibit cross-species activity. TrkB is primarily expressed in the nervous system. However, low levels of TrkB expression have also been observed in a wide variety of tissues (pancreas, kidneys, ovary) outside the nervous system.

Item	Value
Long Name	Neurotrophic Tyrosine Kinase Receptor B
Entrez Gene IDs	4915 (Human); 18212 (Mouse); 25054 (Rat)
Alternate Names	BDNF/NT-3 growth factors receptor, EC 2.7.10, EC 2.7.10.1, GP145-TrkB, NTRK2, Neurotrophic tyrosine kinase receptor type 2, TRKBBDNF-tropomyosine receptor kinase B, TrkB, TrkB tyrosine kinase, neurotrophic tyrosine kinase, receptor, type 2, trk-B, tyrosine kinase receptor B

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