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Key Product Details

Species Reactivity

Human

Applications

CyTOF-ready, Flow Cytometry, Neutralization, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 98725

Product Summary for Human FGFR2 Antibody

Immunogen

S. frugiperda insect ovarian cell line Sf 21-derived recombinant human FGF R2 isoforms and Mouse myeloma cell line NS0-derived recombinant human FGF R2 isoforms

Specificity

Detects alpha isoforms (3 Ig-like domains) and beta isoforms (2 Ig-like domains) of human FGF R2 in direct ELISAs and Western blots. In direct ELISAs, this antibody reacts with all isoforms of rhFGF R2 but shows approximately a 4-fold preference for (IIIc) isoforms. Approximately 100% cross-reactivity with recombinant mouse FGF R2, 15% cross-reactivity with recombinant human (rh) FGF RI ( beta isoforms), and no cross-reactivity with rhFGF RI ( alpha isoforms), rhFGF R3, or rhFGF R4 is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human FGFR2 Antibody

FGF R2 alpha Inhibition of FGF acidic-dependent Cell Proliferation and Neutralization by Human FGF R2 Antibody.

FGF R2 alpha Inhibition of FGF acidic-dependent Cell Proliferation and Neutralization by Human FGF R2 Antibody.

Recombinant Human FGF R2a (IIIc) Fc Chimera (Catalog # 712-FR) inhibits Recombinant Human FGF acidic (Catalog # 232-FA) induced proliferation in the NR6R-3T3 mouse fibroblast cell line in a dose-dependent manner (orange line). Inhibition of Recombinant Human FGF acidic (0.3 ng/mL) activity elicited by Recombinant Human FGF R2a (IIIc) Fc Chimera (4 ng/mL) is neutralized (green line) by increasing concentrations of Human FGF R2 Monoclonal Antibody (Catalog # MAB684). The ND50 is typically 1-5 µg/mL in the presence of heparin (10 µg/mL).

Applications for Human FGFR2 Antibody

Application
Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

2.5 µg/106 cells
Sample: Kato III human cell line

Western Blot

1 µg/mL
Sample: Recombinant Human FGF R2 alpha (IIIb) Fc Chimera (Catalog # 663-FR)
Recombinant Human FGF R2 alpha (IIIc) Fc Chimera (Catalog # 712-FR)
Recombinant Human FGF R2 beta (IIIb) Fc Chimera (Catalog # 665-FR)
Recombinant Human FGF R2 beta (IIIc) Fc Chimera (Catalog # 684-FR)

Neutralization

Measured by its ability to neutralize FGF R2 alpha-mediated inhibition of proliferation in the NR6R‑3T3 mouse fibroblast cell line. The Neutralization Dose (ND50) is typically 1-5 µg/mL in the presence of 4 ng/mL Recombinant Human FGF R2 alpha (IIIc) Fc Chimera, 0.3 ng/mL Recombinant Human FGF acidic, and 10 µg/mL heparin.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Published Applications

Read 5 publications using MAB684 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
Size / Price
Qty
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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: FGFR2

FGF R2 is a transmembrane tyrosine kinase that functions as a receptor for several FGF family proteins. The extracellular domain includes three ( alpha isoforms) or two ( beta isoforms) Ig-like domains. Alternative exon usage results in IIIb or IIIc isoforms that differ in their membrane proximal Ig-like domain.

Long Name

Fibroblast Growth Factor Receptor 2

Alternate Names

CD332, FGF R2

Entrez Gene IDs

2263 (Human); 14183 (Mouse)

Gene Symbol

FGFR2

Additional FGFR2 Products

Product Documents for Human FGFR2 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human FGFR2 Antibody

For research use only

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Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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