Human CCR8 Antibody

Catalog # MAB1429 | R&D Systems, Inc. a Bio-Techne Brand
Clone 191704 was used by HLDA to establish CD designation
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MAB1429-100
MAB1429-SP
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Key Product Details

Species Reactivity

Human

Applications

CyTOF-ready, Flow Cytometry, Neutralization

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG2B Clone # 191704

Product Summary for Human CCR8 Antibody

Immunogen

BaF3 mouse pro-B cell line transfected with human CCR8
Met1-Leu355
Accession # P51685

Specificity

Detects human CCR8. Neutralizes the bioactivity of human CCR8 and specifically stains human CCR8-transfected cell lines but not with the parent cell lines.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2B

Scientific Data Images for Human CCR8 Antibody

Detection of CCR8 antibody in Human Blood antibody by Flow Cytometry.

Detection of CCR8 in Human Blood by Flow Cytometry.

Human peripheral blood cells were stained with (A) Rat Anti-Human CCR8 Monoclonal Antibody (Catalog # MAB1429) or (B) isotype control antibody (Catalog # MAB0061) followed by anti-Rat IgG APC-conjugated Secondary Antibody (Catalog # F0113) and Mouse anti-Human CD14 PE-conjugated Monoclonal Antibody (Catalog # FAB3832P). View our protocol for Staining Membrane-associated Proteins.
Chemotaxis Induced by CCL1/I‑309 and Neutralization by Human CCR8 Antibody.

Chemotaxis Induced by CCL1/I‑309 and Neutralization by Human CCR8 Antibody.

Recombinant Human CCL1/I-309 (Catalog # 272-I) chemoattracts the BaF3 mouse pro-B cell line transfected with human CCR8 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Human CCL1/I-309 (20 ng/mL) is neutralized (green line) by increasing concentrations of Rat Anti-Human CCR8 Monoclonal Antibody (Catalog # MAB1429). The ND50 is typically 0.01-0.05 µg/mL.

Applications for Human CCR8 Antibody

Application
Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

0.25 µg/106 cells
Sample: Human peripheral blood cells

Neutralization

Measured by its ability to neutralize CCL1/I‑309/TCA‑3-induced chemotaxis in the BaF3 mouse pro‑B cell line transfected with human CCR8. The Neutralization Dose (ND50) is typically 0.01-0.05 µg/mL in the presence of 20 ng/mL Recombinant Human CCL1/I‑309/TCA‑3.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 1 review rated 1 using MAB1429 in the following applications:

Formulation, Preparation and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Reconstitution Buffer Available:
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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CCR8

CCR8 (C-C chemokine receptor 8; previously called CKR-L1 or TER1 and designated CD198) is a 41-43 kDa member of the GPCR 1 family of transmembrane proteins.  CCR8 is expressed on vascular smooth muscle cells, monocytes, eosinophils, peritoneal macrophages, thymocytes, CD8+ T cells, Langerhans cells and neurons.  CCL1/TCA3 and vMIP-1 are known agonists for CCR8.  Human CCR8 is a 7-transmembrane protein that is 355 amino acids (aa) in length.  It contains a 35 aa N-terminal extracellular domain plus a 51 aa C-terminal cytoplasmic tail. Over aa sequences 1-33 and 92-105 collectively, human and mouse CCR8 share 64% aa sequence identity.

Alternate Names

CCR8, CD198, CDw198, ChemR1, CKRL1, GPRCL1, TER-1

Entrez Gene IDs

1237 (Human); 12776 (Mouse)

Gene Symbol

CCR8

UniProt

P51685

Product Documents for Human CCR8 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Human CCR8 Antibody

For research use only

Citations for Human CCR8 Antibody (3)

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Customer Reviews for Human CCR8 Antibody (1)

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  • Human CCR8 Antibody
    Name: Peter Brams
    Application: Flow Cytometry
    Sample Tested: CHO Chinese hamster ovary cell line
    Species: Rat , Human and Human CCR8 in hamster and human cells
    Verified Customer | Posted 11/16/2019
    We ran standard FACS staining on human CCR8 transfected CHO and HEK293 cells. The antibody did not bind specifically, at all. Standard controls, including other antibodies, worked. We did the experiment with and without a N'terminal tag; same results.
    Human CCR8 Antibody MAB1429

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FAQs for Human CCR8 Antibody

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  • Q: Are there special considerations for Flow Cytometry using Human CCR8 Antibody, Catalog # MAB1429 and FAB1429?

    A: The staining with this antibody is temperature dependent;  it will stain at 4 C and room temperature, however, staining is stronger at 37 C.

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Reconstitution Calculator

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