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GSDMDC1 Antibody

Catalog # NBP2-80427 | Novus Biologicals a Bio-Techne Brand
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NBP2-80427

Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1 mg/ml

Product Summary for GSDMDC1 Antibody

Immunogen

Recombinant protein within human GSDMDC1 aa 100-300. (SwissProt: P57764 Human; SwissProt: Q9D8T2 Mouse; SwissProt: Q9D8T2 Mouse)

Localization

Cytosol. Secreted. Plasma membrane.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

53 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for GSDMDC1 Antibody

Western Blot: GSDMDC1 Antibody [NBP2-80427] - Analysis of Gasdermin D (N terminal) on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: SiHa cell lysateLane 2: human kidney tissue lysate
Immunocytochemistry/Immunofluorescence: GSDMDC1 Antibody [NBP2-80427] - Staining of Gasdermin D in SiHa cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ER1901-37, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
Immunohistochemistry-Paraffin: GSDMDC1 Antibody [NBP2-80427] - Analysis in Mouse colon tissue using GSDMDC1 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with

Applications for GSDMDC1 Antibody

Application
Recommended Usage

Flow Cytometry

1:50-1:100

Immunocytochemistry/ Immunofluorescence

1:50-1:100

Immunohistochemistry-Paraffin

1:50-1:200

Western Blot

1:500-1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Published Applications

Read 4 publications using NBP2-80427 in the following applications:

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH7.4), 0.2% BSA, 50% Glycerol

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: GSDMDC1

Gasdermin D is a member of the gasdermin family. Members of this family appear to play a role in regulation ofepithelial proliferation. Gasdermin D has been suggested to act as a tumor suppressor. Alternatively splicedtranscript variants have been described. (provided by RefSeq)

Long Name

Gasdermin-D

Alternate Names

DFNA5L, FKSG10, GSDMD, hGSDMD-CTD)

Gene Symbol

GSDMD

Product Documents for GSDMDC1 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for GSDMDC1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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