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CCR5 Antibody (T21/8) - BSA Free

Catalog # NBP1-43335 | Novus Biologicals a Bio-Techne Brand

Key Product Details

Species Reactivity

Human

Applications

ELISA, Flow Cytometry, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # T21/8

Format

BSA Free

Concentration

0.5 mg/ml

Product Summary for CCR5 Antibody (T21/8) - BSA Free

Immunogen

The immunogen for this antibody was CCR5.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Scientific Data Images for CCR5 Antibody (T21/8) - BSA Free

Flow Cytometry: CCR5 Antibody (T21/8) [NBP1-43335] - Analysis using the PE conjugate of NBP1-43335. Staining of normal human peripheral blood cells with Anti-Human CD3 APC and Mouse IgG1 kappa Isotype Control PE (left) or Anti-Human CD195 (CCR5) PE (right). Cells in the lymphocyte gate were used for analysis.
Flow Cytometry: CCR5 Antibody (T21/8) [NBP1-43335] - Staining of normal human peripheral blood cells with 0.25 ug of Mouse IgG1 kappa Isotype Control Purified (open histogram) or 0.25 ug of Anti-Human CD195 (CCR5) Purified (filled histogram) followed by Anti-Mouse IgG Biotin and Streptavidin PE. Cells in the lymphocyte gate were used for analysis.
Flow Cytometry: CCR5 Antibody (T21/8) [NBP1-43335] - Staining of normal human peripheral blood cells with Anti-Human CD3 FITC and 0.25 ug of Mouse IgG1 kappa Isotype Control BIOTIN (left) or 0.25 ug of Anti-Human CD195 (CCR5) BIOTIN (right) followed by Streptavidin PE. Cells in the lymphocyte gate were used

Applications for CCR5 Antibody (T21/8) - BSA Free

Application
Recommended Usage

ELISA

1:100-1:2000

Flow Cytometry

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Paraffin

1:10-1:500

Immunoprecipitation

1:10-1:500

Western Blot

1:100-1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Published Applications

Read 1 publication using NBP1-43335 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

PBS (pH 7.2)

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: CCR5

The T21/8 monoclonal antibody reacts with the amino terminus of human CCR5 (CD195), an approximately 45 kDa member of the G protein-coupled receptor (GPCR) superfamily that binds chemokines including MIP-1alpha, MIP-1beta and RANTES. Human CCR5 is expressed on resting T cells with a memory/effector phenotype, monocytes, macrophages and immature dendritic cells. CCR5 functions as a receptor to direct the chemotaxis of lymphocytes, and is the primary co-receptor for macrophage-tropic Human Immunodeficiency Virus Type 1 (HIV-1), which binds CCR5 through gp120. Binding of the T21/8 monoclonal antibody displays donor variability and may be affected by sub-optimal staining conditions.

Alternate Names

CCR5, CD195

Entrez Gene IDs

1234 (Human)

Gene Symbol

CCR5

UniProt

Additional CCR5 Products

Product Documents for CCR5 Antibody (T21/8) - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for CCR5 Antibody (T21/8) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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