CCAR1 Antibody - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NB100-258

Novus Biologicals, part of Bio-Techne
Discontinued Product
NB100-258 has been discontinued. View all CCAR1 products.

Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Immunoprecipitation, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

1.0 mg/ml

View all CCAR1 Antibodies »

Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residue 700 and 750 of human Cell-Cycle and Apoptosis Regulatory Protein 1 using the numbering given in entry NP_060707.2 (GeneID 55749).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for CCAR1 Antibody - BSA Free

Western Blot: CCAR1 Antibody [NB100-258]

Western Blot: CCAR1 Antibody [NB100-258]

Western Blot: CCAR1 Antibody [NB100-258] - Detection of Human CCAR1 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa and 293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-CCAR1 antibody NB100-258 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Western Blot: CCAR1 Antibody [NB100-258]

Western Blot: CCAR1 Antibody [NB100-258]

Western Blot: CCAR1 Antibody [NB100-258] - Detection of Human DIS on HeLa whole cell lystate using NB100-258. Controls: Normal IgG or another rabbit anti-DIS.

Applications for CCAR1 Antibody - BSA Free

Application
Recommended Usage

Immunoprecipitation

1-4 ug/mg lysate

Western Blot

1:2000-1:10000
Application Notes
By Western blot, this antibody recognizes bands around 150 kDa representing DIS. Also useful for Immunoprecipitation.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: CCAR1

CCAR1 was identified as a perinuclear phosphoprotein that regulates apoptosis signals in response to chemotherapeutic agents. Induction of apoptosis by CCAR1 involves the epidermal growth factor receptor (EGFR), mitogen-activated protein kinase pathway and caspase-9 activation. CARP-1 has also been demonstrated as a suppressor of human breast cancer growth. CCAR1 is also known as cell division cycle and apoptosis regulator protein 1, cell cycle and apoptosis regulatory protein 1, CARP-1, death inducer with SAP domain, DIS, MGC44628, and RP11-437A18.1.

Alternate Names

CARP-1, CARP1MGC44628, Cell cycle and apoptosis regulatory protein 1, cell division cycle and apoptosis regulator 1, cell division cycle and apoptosis regulator protein 1, Death inducer with SAP domain, DIS, FLJ10590, RP11-437A18.1

Entrez Gene IDs

55749 (Human)

Gene Symbol

CCAR1

UniProt

NP_060707.2 (Human)

Additional CCAR1 Products

Product Documents for CCAR1 Antibody - BSA Free

Product Datasheets

Certificate of Analysis

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Product Specific Notices for CCAR1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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