Beta-Amylase Antibody - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NB600-857
Key Product Details
Species Reactivity
Validated:
Plant
Cited:
P. vulgaris
Applications
Validated:
Western Blot, ELISA
Cited:
Western Blot, SDS-Page
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
Concentration
LYOPH mg/ml
Product Specifications
Immunogen
Beta-Amylase [Sweet Potato] (Uniprot: P10537)
Reactivity Notes
Cross reactivity against Beta Amylase from other sources may occur but has not been specifically determined.
Localization
Cytoplasmic
Specificity
Cross reactivity against b Amylase from other sources may occur but has not been specifically determined.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Description
This antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum as well as purified and partially purified b Amylase [Sweet Potato]
Store vial at 4C prior to restoration. For extended storage aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.
Store vial at 4C prior to restoration. For extended storage aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.
Applications for Beta-Amylase Antibody - BSA Free
Application
Recommended Usage
ELISA
1:5000-1:25000
Western Blot
1:500-1:3000
Application Notes
This product has been tested by western blot and is suitable to be assayed against 1.0 ug of b Amylase [Sweet Potato] in a standard ELISA using Peroxidase conjugated Affinity Purified anti-Rabbit IgG [H&L] (Goat) and (ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1:4,000 to 1:16,000 of the reconstitution concentration is suggested for this product.
Formulation, Preparation, and Storage
Purification
Multi-step
Reconstitution
Reconstitute with 100 ul deionized water (or equivalent)
Formulation
Lyophilized from 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Format
BSA Free
Preservative
0.01% Sodium Azide
Concentration
LYOPH mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store lyophilized antibody at 4C. Aliquot reconstituted liquid and store at -20C. Avoid freeze-thaw cycles.
Background: Beta-Amylase
Alternate Names
14 alpha D glucan maltohydrolase, 1-4 alpha D glucan maltohydrolase, AMYB, BMY1
UniProt
Additional Beta-Amylase Products
Product Documents for Beta-Amylase Antibody - BSA Free
Product Specific Notices for Beta-Amylase Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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