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Total Collagen Assay Kit (Colorimetric)

Catalog # NBP2-59748 | Novus Biologicals a Bio-Techne Brand
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NBP2-59748

Key Product Details

Sample Type & Volume Required Per Well

Mammalian tissues, protein/peptide hydrolysates, serum, urine

Product Specifications

Kit Type

Assay Kit (Colorimetric)

Reactivity

Mammal

Description

This kit is a quick and convenient protocol for detection of small amounts of collagen in a variety of biological samples. The assay is based upon hydrolysis of samples to yield free Hyp, which is oxidized and reacted to give a brightly-colored chromophore with an absorbance peak at 560 nm. The assay employs a proprietary acidic developer solution to accurately measure collagen in hydrolysates without the use of hazardous perchloric acid, obviating the need for any special handling and waste-disposal protocols. The assay can detect a minimum of 0.5 ug collagen per well in a 96-well format and generates colorimetric results that are virtually identical to those of the classical perchloric acid-based method.



This product is manufactured by Abcam and distributed by Novus Biologicals. (Abcam Catalog Number: ab222942)

Scientific Data Images for Total Collagen Assay Kit (Colorimetric)

Total Collagen Colorimetric Assay Kit [NBP2-59748]
Total Collagen Colorimetric Assay Kit [NBP2-59748]

Kit Contents for Total Collagen Assay Kit (Colorimetric)

  • Chloramine T Concentrate
  • Collagen I Standard (3 mg/ml)
  • Developer Solution
  • DMAB Concentrate (in DMSO)
  • Microplate Sealing Film
  • Oxidation Buffer

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C in the dark. Avoid freeze-thaw cycles.

Background: Total Collagen

Collagen is the most abundant protein in mammals, accounting for nearly 30% of body protein content. Collagen is an extracellular scaffolding protein found in skin, tendons, bone, cartilage, ligaments and muscles, amongst other tissues. There are more than twenty different characterized types of collagen, but the fibrilous types I, II and III comprise more than 90% of the total collagen in mammals. Collagen has a triple helical structure, consisting of three 'preprocollagen' strands with a repeating Gly-X-Hyp tripeptide sequence, where X is any amino acid and Hyp is hydroxyproline, a post-translationally modified amino acid formed from proline by the enzyme prolyl-4-hydroxylase. In animals, hydroxyproline is found almost entirely in collagen (which contains approximately 12-14% hydroxyproline by mass) and contributes to its structural rigidity and high tensile strength. Hydroxyproline is thus a direct measure of the amount of collagen present in tissues. Many diseases are believed to affect collagen turnover, including tumor invasion and metastasis, rheumatoid arthritis, cardiopulmonary fibrosis and muscular dystrophy.

Product Documents for Total Collagen Assay Kit (Colorimetric)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Total Collagen Assay Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Kits are guaranteed for 6 months from date of receipt.

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