Immunohistochemistry (IHC) HandbookE-books
Immunohistochemistry (IHC) is a widely used immunostaining technique using antibodies to detect an antigen of interest in the cells of a fixed tissue section and provides semi-quantitative data about target protein expression, distribution, and localization.
Our recently updated handbook offers a comprehensive introduction to IHC that is well-suited for researchers new to the technique and experienced scientists looking for a quick refresher.
Download our NEW handbook and learn about:
- Different variables to consider when performing an IHC experiment
- Multiplexing IHC
- RNA-Protein co-detection and the benefits of in situ hybridization (ISH)-IHC
- Standard IHC workflow including sample preparation and fixation, antigen retrieval, blocking non-specific binding, antibody selection, and detection methods
- Controls for IHC
- Easy-to-follow IHC staining protocols (chromogenic and fluorescent)
- Troubleshooting tips
- Buffer and reagent recipes
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