g-linked ATP agarose resin: Supplemental Kits and Reagents

ATP linked to a support via its g-phosphate has been used for affinity purification of kinases, heat-shock proteins and other ATP-binding proteins. KinaseBind is a g-phosphate-linked ATP resin with a long hydrophillic spacer that facilitates molecular interactions with biomolecules. Linkage of ATP via the terminal phosphate means that the immobilised ligand cannot be removed by phosphatases in crude extracts.