X-Gal

Tocris Bioscience, part of Bio-Techne | Catalog # 5510

Used in cloning procedures with IPTG
Discontinued Product
5510 has been discontinued. View all Buffers, Chelators and Reagents products.

Key Product Details

Description

Used in cloning procedures with IPTG

View all Buffers, Chelators and Reagents Supplemental Kits and Reagents »

Product Description

Chromogenic substrate of β-Galactosidase that produces blue color. Used in combination with IPTG (Cat. No. 5509) for detection of β-Galactosidase activity in bacterial colonies.

Product Specifications

Molecular Weight

408.63

Formula

C14H15BrClNO6

Storage

Store at -20°C

Purity

≥98% (HPLC)

Chemical Name

5-Bromo-4-chloro-1H-indoll-3-yl-β-D-galactopyranoside

CAS Number

7240-90-6

PubChem ID

65181

InChI Key

OPIFSICVWOWJMJ-AEOCFKNESA-N

SMILES

ClC1=C(C(O[C@H]3[C@H](O)[C@@H](O)[C@@H](O)[C@@H](CO)O3)=CN2)C2=CC=C1Br

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Solubility

Solvent Max Conc. mg/mL Max Conc. mM
Solubility
DMSO 40.86 100

Preparing Stock Solutions for X-Gal

The following data is based on the product molecular weight 408.63

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which all affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:

Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 2.45 mL 12.24 mL 24.47 mL
5 mM 0.49 mL 2.45 mL 4.89 mL
10 mM 0.24 mL 1.22 mL 2.45 mL
50 mM 0.05 mL 0.24 mL 0.49 mL

Calculators

Molarity Calculator

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*When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and CoA (available online).

Dilution Calculator

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Reconstitution Calculator

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Product Documents for X-Gal

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for X-Gal

For research use only

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