Sulfo-NHS-LC-Biotin
Tocris Bioscience, part of Bio-Techne | Catalog # 7302
Amine reactive biotinylation reagent, cell impermeable
Key Product Details
Description
Amine reactive biotinylation reagent, cell impermeable
Product Description
Sulfo-NHS-LC-Biotin is a water-soluble amine reactive biotinylation reagent used to attach biotin to primary amines. Commonly used to label proteins and antibodies. Does not penetrate membrane. Reactive in pH 7.0-9.0, solubility 10 mg/mL in water and DMF.Product Specifications
Molecular Weight
556.58
Formula
C20H29N4NaO9S2
Storage
Store at -20°C
Chemical Name
Sodium 2,5-dioxo-1-((6-(5-((3aS,4S,6aR)-2-oxohexahydro-1H-thieno[3,4-d]imidazol-4-yl)pentanamido)hexanoyl)oxy)pyrrolidine-3-sulfonate
CAS Number
191671-46-2
PubChem ID
71571497
InChI Key
JJGWLCLUQNFDIS-GTSONSFRSA-M
SMILES
[H][C@]12CS[C@H]([C@]1(NC(N2)=O)[H])CCCCC(NCCCCCC(ON3C(CC(S([O-])(=O)=O)C3=O)=O)=O)=O.[Na+]
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Solubility
| Solvent | Max Conc. mg/mL | Max Conc. mM | |
|---|---|---|---|
| Solubility | |||
| water | 55.66 | 100 | |
| DMSO | 55.66 | 100 |
Preparing Stock Solutions
for Sulfo-NHS-LC-Biotin
The following data is based on the product molecular weight 556.58
Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which all affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 1 mM | 1.80 mL | 8.98 mL | 17.97 mL |
| 5 mM | 0.36 mL | 1.80 mL | 3.59 mL |
| 10 mM | 0.18 mL | 0.90 mL | 1.80 mL |
| 50 mM | 0.04 mL | 0.18 mL | 0.36 mL |
Calculators
Molarity Calculator
Dilution Calculator
Reconstitution Calculator
Background References
References are publications that support the biological activity of the product.
- Xu and Wegner Multifunctional streptavidin-biotin conjugates with precise stoichiometries. Chem.Sci.
- Karunakaran Butyrophilin-2A1 directly binds germline-encoded regions of the Vγ9Vδ2 TCR and is essential for phosphoantigen sensing. Immunity 2020 PMID: 32155411
- Takayama Long-term drug modification to the surface of mesenchymal stem cells by the avidin-biotin complex method. Sci.Rep 2017 PMID: 29208980
Product Specific Notices for Sulfo-NHS-LC-Biotin
For research use only
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