Proteinase K
Novus Biologicals, part of Bio-Techne | Catalog # NB900-66727
Key Product Details
Applications
Immunohistochemistry
Product Summary for Proteinase K
This 25X Proteinase K solution is specifically formulated to achieve proteolytic digestion of formalin fixed, paraffin embedded tissue sections prior to performing in situ hybridization procedures. Proteolytic digestion of formalin fixed tissues improves the accessibility of target DNA to bioinylated probes, which results in better in situ staining.
Product Specifications
Application Notes
Preparation of Working Solutions
1. Place 100uL Proteinase K solution in a glass tube, add 2.4mL distilled water, and mix.
Protocol Recommendations
1. Optimal digestion conditions will vary depending on fixation conditions and should be optimized by the end user.
2. Incubation of tissue sections for 10-30 minutes at 37C is sufficient for in situ.
1. Place 100uL Proteinase K solution in a glass tube, add 2.4mL distilled water, and mix.
Protocol Recommendations
1. Optimal digestion conditions will vary depending on fixation conditions and should be optimized by the end user.
2. Incubation of tissue sections for 10-30 minutes at 37C is sufficient for in situ.
Formulation, Preparation, and Storage
Formulation
Sodium Azide is included at less than 0.1%. Exact amount not available.
Preservative
Sodium Azide
Concentration
Please see the protocols for proper use of this product. If no protocol is available, contact technical services for assistance.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Proteinase K
Alternate Names
Endopeptidase K, PROK, Tritirachium alkaline proteinase
Additional Proteinase K Products
Product Documents for Proteinase K
Product Specific Notices for Proteinase K
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Support products are guaranteed for 6 months from date of receipt.
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