NAI-N3
Tocris Bioscience, part of Bio-Techne | Catalog # 7003
Reagent for RNA icSHAPE experiments in vivo
Discontinued Product
7003 has been discontinued.
View all General Laboratory Research Reagent products.
Key Product Details
Description
Reagent for RNA icSHAPE experiments in vivo
Product Description
NAI-N3 is a probe for icSHAPE (in vivo click selective 2'-hydroxyl acylation and profiling experiment). Acylates the 2'-OH in ssRNA. Enables RNA structure profiling in vivo and ex vivo for all four bases. NAI-N3 can be used for site-specific RNA acylation using the RAIL (RNA Acylation at Induced Loops) method or in Apta-Seq to discover new aptamers in selected pools.Product Specifications
Molecular Weight
228.22
Formula
C10H8N6O
Storage
Store at -20°C
Chemical Name
[2-(Azidomethyl)-3-pyridinyl]-1H-imidazol-1-ylmethanone
CAS Number
1612756-29-2
PubChem ID
131704490
InChI Key
QPSQVTFTPHUCEH-UHFFFAOYSA-N
SMILES
[N-]=[N+]=NCC1=C(C(N2C=CN=C2)=O)C=CC=N1
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Solubility
| Solubility | Soluble in DMSO (supplied pre-dissolved in DMSO, 1M) |
Calculators
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Background References
References are publications that support the biological activity of the product.
- Abdelsayed Multiplex aptamer discovery through Apta-Seq and its application to ATP aptamers derived from human-genomic SELEX. ACS Chem.Biol. 2017 PMID: 28661647
- Lee Comparison of SHAPE reagents for mapping RNA structures inside living cells. RNA 2017 PMID: 27879433
- Xiao Site-selective RNA functionalization via DNA-induced structure. J.Am.Chem.Soc. 2020 PMID: 32865995
- Spitale Structural imprints in vivo decode RNA regulatory mechanisms. Nature 2015 PMID: 25799993
Product Specific Notices for NAI-N3
For research use only
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